FG Angewandte Biochemie

17 Items

Recent Submissions
Coxsackievirus B3—Its Potential as an Oncolytic Virus

Geisler, Anja ; Hazini, Ahmet ; Heimann, Lisanne ; Kurreck, Jens ; Fechner, Henry (2021-04-21)

Oncolytic virotherapy represents one of the most advanced strategies to treat otherwise untreatable types of cancer. Despite encouraging developments in recent years, the limited fraction of patients responding to therapy has demonstrated the need to search for new suitable viruses. Coxsackievirus B3 (CVB3) is a promising novel candidate with particularly valuable features. Its entry receptor, ...

Clean Bioprinting – Fabrication of 3D Organ Models Devoid of Animal Components

Berg, Johanna ; Kurreck, Jens (2021-04-20)

Bioprinting is a rapidly developing technology that enables the exact positioning of living cells embedded in bio-materials in precise spatial arrangements to fabricate engineered tissues and organs. While the ultimate goal of bio­printing approaches is to produce organs for transplantation purposes, bioprinted organ models also hold great potential for research purposes to serve as alternative...

Developmental Landscape of Potential Vaccine Candidates Based on Viral Vector for Prophylaxis of COVID-19

Bezbaruah, Rajashri ; Borah, Pobitra ; Kakoti, Bibhuti Bhushan ; Al-Shar’I, Nizar A. ; Chandrasekaran, Balakumar ; Jaradat, Da’san M. M. ; Al-Zeer, Munir A. ; Abu-Romman, Saeid (2021-04-15)

Severe acute respiratory syndrome coronavirus 2, SARS-CoV-2, arose at the end of 2019 as a zoonotic virus, which is the causative agent of the novel coronavirus outbreak COVID-19. Without any clear indications of abatement, the disease has become a major healthcare threat across the globe, owing to prolonged incubation period, high prevalence, and absence of existing drugs or vaccines. Developm...

Verbesserung der Zytotoxizität eines TRAIL-exprimierenden onkolytischen Adenovirus in Melanomzellen durch Knockdown des antiapoptotischen Proteins Mcl-1

Tolksdorf, Beatrice Dorothea Friederike (2021)

Die Inzidenz des malignen Melanoms hat in den letzten Jahrzehnten zugenommen. Weltweit wird jedes Jahr bei ungefähr 300.000 Patienten ein Melanom diagnostiziert. Das Melanom gehört damit zu den zehn häufigsten Tumorarten in Nordamerika, Westeuropa und Ozeanien. In den letzten Jahren hat die Zulassung von selektiven Inhibitoren für MAP-Kinasen BRAF und MEK sowie von Immuncheckpoint-Inhibitoren, ...

3D-bioprinted HepaRG cultures as a model for testing long term aflatoxin B1 toxicity in vitro

Schmidt, Konrad ; Berg, Johanna ; Roehrs, Viola ; Kurreck, Jens ; Al-Zeer, Munir A. (2020-11-21)

In recent years 3D-bioprinting technology has been developed as an alternative to animal testing. It possesses a great potential for in vitro testing as it aims to mimic human organs and physiology. In the present study, an alginate-gelatin-Matrigel based hydrogel was used to prepare 3D-bioprinted HepaRG cultures using a pneumatic extrusion printer. These 3D models were tested for viability and...

Mcl‐1 targeting strategies unlock the proapoptotic potential of TRAIL in melanoma cells

Sarif, Zina ; Tolksdorf, Beatrice ; Fechner, Henry ; Eberle, Jürgen (2020-09-04)

TNF‐related apoptosis‐inducing ligand (TRAIL) induces apoptosis selectively in cancer cells. For melanoma, the targeting of TRAIL signaling appears highly attractive, due to pronounced TRAIL receptor expression in tumor tissue. However, mechanisms of TRAIL resistance observed in melanoma cells may limit its clinical use. The Bcl‐2 family members are critical regulators of cell‐intrinsic apoptot...

The dynamic proteome of Influenza A virus infection identifies species- and strain-specific control of M segment RNA splicing

Bogdanow, Boris (2020)

All viruses hijack host cells to replicate. To this end, they have to interact with cellular factors at all stages of the replication cycle. Since these host cell factors differ markedly between species, viruses typically need to adapt in order to cross a species barrier. Advances in high-throughput sequencing have provided insights into the extraordinary diversity of viruses and genomic determ...

MiR‐375‐mediated suppression of engineered coxsackievirus B3 in pancreatic cells

Pryshliak, Markian ; Hazini, Ahmet ; Knoch, Klaus ; Dieringer, Babette ; Tolksdorf, Beatrice ; Solimena, Michele ; Kurreck, Jens ; Pinkert, Sandra ; Fechner, Henry (2019-11-07)

Coxsackievirus B3 (CVB3) has potential as a new oncolytic agent for the treatment of cancer but can induce severe pancreatitis. Here, we inserted target sequences of the microRNA miR‐375 (miR‐375TS) into the 5′ terminus of the polyprotein encoding sequence or into the 3′UTR of the CVB3 strain rCVB3.1 to prevent viral replication in the pancreas. In pancreatic EndoC‐βH1 cells expressing miR‐375 ...

Entwicklung eines dreidimensional gedruckten humanisierten Lebermodells als Modellsystem für virale Infektions- und Transduktionsversuche

Hiller, Thomas (2019)

Die Leber ist das zentrale Stoffwechselorgan des menschlichen Organismus und ist verantwortlich für eine Vielzahl essentieller Stoffwechselvorgänge. Adenovirale Infektionen der Leber können schwerwiegenden Gewebsschädigungen verursachen mit pathologischen Konsequenzen für den menschlichen Organismus. Bestehende antiadenovirale Therapien sind oftmals nur begrenzt wirksam und können zudem starke ...

Verbesserung des CVB3-Maus-Myokarditismodells durch die Entwicklung pankreasattenuierter Coxsackieviren

Pryshliak, Markian (2019)

Das Coxsackievirus B3 (CVB3)-Maus-Myokarditismodell wird standardmäßig zur Untersuchung der Virus-induzierten Myokarditis verwendet. Entgegen der CVB3-Infektion im Menschen stellt im murinen Organismus nicht das Herz, sondern das Pankreas das für die CVB3-Infektion suszeptibelste Organ dar. Verschiedene klinische Symptome, wie der starke Gewichtsverlust, das schmerzhafte Hochziehen des Bauches ...

Impact of TiO2 nanoparticles on the aquatic environment: Investigation of cyanobacterial toxin adsorption and oxidative stress mediated nanotoxicity towards the submerged aquatic macrophyte Hydrilla verticillata

Spengler, Annette (2019)

Manufactured TiO2-nanoparticles (TiO2-NPs) are the most commonly employed metal oxide-based NPs. Due to the greater surface area per unit volume compared with their respective microscale counterparts, TiO2-NPs might exhibit physicochemical properties which affect the toxicity potential of the normally inert material. Taking into account the increasing production volumes of TiO2-NPs, they will i...

Phthalocyanine-fullerene dyads and DNA interstrand cross-linking on surface

Véliz Montes, Cinthya Yamila (2018)

Nanotechnology deals with structures and components of nanoscale size in order to create new properties or to improve significantly their physical, chemical and biological properties, processes and phenomena. Many efforts of molecular nanotechnology are directed to design artificial nanostructures on surfaces due to their potential applications in nanodevices and biosensors. This thesis include...

Generation of a 3D Liver Model Comprising Human Extracellular Matrix in an Alginate/Gelatin-Based Bioink by Extrusion Bioprinting for Infection and Transduction Studies

Hiller, Thomas ; Berg, Johanna ; Elomaa, Laura ; Röhrs, Viola ; Ullah, Imran ; Schaar, Katrin ; Dietrich, Ann-Christin ; Al-Zeer, Munir A. ; Kurtz, Andreas ; Hocke, Andreas C. ; Hippenstiel, Stefan ; Fechner, Henry ; Weinhart, Marie ; Kurreck, Jens (2018-10-12)

Bioprinting is a novel technology that may help to overcome limitations associated with two-dimensional (2D) cell cultures and animal experiments, as it allows the production of three-dimensional (3D) tissue models composed of human cells. The present study describes the optimization of a bioink composed of alginate, gelatin and human extracellular matrix (hECM) to print human HepaRG liver cell...

Analysis of metabolic pathways controlling cAMP-regulated aquaporin-2 trafficking in renal principal cells

Schulz, Maike (2018)

One of the main effectors of the second messenger cyclic adenosine monophosphate (cAMP) is the ubiquitously expressed serine/threonine protein kinase, cAMP-dependent protein kinase (PKA). Binding of cAMP to the regulatory subunits of PKA results in a conformational change and the release of the catalytic subunits to phosphorylate nearby targets. To ensure spatially and temporally controlled pho...

Optimization of cell-laden bioinks for 3D bioprinting and efficient infection with influenza A virus

Berg, Johanna ; Hiller, Thomas ; Kissner, Maya S. ; Qazi, Taimoor H. ; Duda, Georg N. ; Hocke, Andreas C. ; Hippenstiel, Stefan ; Elomaa, Laura ; Weinhart, Marie ; Fahrenson, Christoph ; Kurreck, Jens (2018-09-17)

Bioprinting is a new technology, which arranges cells with high spatial resolution, but its potential to create models for viral infection studies has not yet been fully realized. The present study describes the optimization of a bioink composition for extrusion printing. The bioinks were biophysically characterized by rheological and electron micrographic measurements. Hydrogels consisting of ...

Development of recombinant oncolytic Coxsackievirus B3 for treatment of colorectal carcinoma

Hazini, Ahmet (2018)

Colorectal cancer is one of the most death related cancer type in worldwide. Conventional cancer therapeutics show curable effect only in the patients at early stage of the disease. In case of the diagnosis at late stages, the survival rate decreases dramatically up to 10%. Therefore, novel cancer therapeutics are urgently needed. Oncolytic virotherapy is a new treatment approach which exploits...

Hepatitis E virus superinfection and clinical progression in hepatitis B patients

Hoan, Nghiem Xuan ; Tong, Hoang Van ; Hecht, Nicole ; Sy, Bui Tien ; Marcinek, Patrick ; Meyer, Christian G. ; Song, Le Huu ; Toan, Nguyen Linh ; Kurreck, Jens ; Kremsner, Peter G. ; Bock, C-Thomas ; Velavan, Thirumalaisamy P. (2015)

Hepatitis E virus (HEV) infection may cause acute hepatitis and lead to hepatic failure in developing and developed countries. We studied HEV seroprevalences in patients with hepatitis B virus (HBV) infection to understand the consequences of HEV superinfection in a Vietnamese population. This cross-sectional study was conducted from 2012 to 2013 and included 1318 Vietnamese patients with HBV-r...