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Main Title: G9a/GLP complex maintains imprinted DNA methylation in embryonic stem cells
Author(s): Zhang, Tuo
Termanis, Ausma
Özkan, Burak
Bao, Xun X.
Culley, Jayne
de Lima Alves, Flavia
Rappsilber, Juri
Ramsahoye, Bernard
Stancheva, Irina
Type: Article
Language Code: en
Abstract: DNA methylation at imprinting control regions (ICRs) is established in gametes in a sex-specific manner and has to be stably maintained during development and in somatic cells to ensure the correct monoallelic expression of imprinted genes. In addition to DNA methylation, the ICRs are marked by allele-specific histone modifications. Whether these marks are essential for maintenance of genomic imprinting is largely unclear. Here, we show that the histone H3 lysine 9 methylases G9a and GLP are required for stable maintenance of imprinted DNA methylation in embryonic stem cells; however, their catalytic activity and the G9a/GLP-dependent H3K9me2 mark are completely dispensable for imprinting maintenance despite the genome-wide loss of non-imprinted DNA methylation in H3K9me2-depleted cells. We provide additional evidence that the G9a/GLP complex protects imprinted DNA methylation by recruitment of de novo DNA methyltransferases, which antagonize TET dioxygenass-dependent erosion of DNA methylation at ICRs.
Issue Date: 24-Mar-2016
Date Available: 1-Jun-2018
DDC Class: 570 Biowissenschaften; Biologie
610 Medizin und Gesundheit
Subject(s): DNA methylation
imprinting control regions
histone modifications
somatic cells
embryonic stem cells
DNA methyltransferases
Journal Title: Cell Reports
Publisher: Elsevier
Publisher Place: New York, NY
Volume: 15
Issue: 1
Publisher DOI: 10.1016/j.celrep.2016.03.007
Page Start: 77
Page End: 85
ISSN: 2211-1247
Appears in Collections:FG Bioanalytik » Publications

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