Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-7202
Main Title: Surface plasmon resonance based sensor for the detection of glycopeptide antibiotics in milk using rationally designed nanoMIPs
Author(s): Altintas, Zeynep
Type: Article
Language Code: en
Abstract: Glycopeptide antibiotics are known as the last resort for the treatment of serious infections caused by Gram-positive bacteria. The use of milk products contaminated with these antibiotic residues leads to allergic reactions and sensitivity in human. Also, long-term consumption of milk products containing low levels of these antibiotics may cause the relevant bacteria to build up resistance to these last resort antibiotics. Sensitive, rapid and effective quantification and monitoring systems play a key role for their determination in milk products. Hence, molecularly imprinted nanostructures were rationally designed in this work to produce high affinity synthetic receptors to be coupled with a surface plasmon resonance sensor for the analysis of glycopeptide antibiotics in milk samples. The nanoMIP-SPR sensor enabled vancomycin quantification with the LODs of 4.1 ng mL−1 and 17.7 ng mL−1 using direct and competitive assays, respectively. The recoveries rates for two sensor methods ranged in 85–110% with RSDs below 7%. The affinity between the nanoMIP receptors and the target molecule (dissociation constant: 1.8 × 10−9 M) is mostly superior to natural receptors and other synthetic receptors. Unlike other methods commonly employed for the detection of milk contaminants this approach is extremely simple, fast and robust, and do not require pre-sample treatment.
URI: https://depositonce.tu-berlin.de//handle/11303/8038
http://dx.doi.org/10.14279/depositonce-7202
Issue Date: 25-Jul-2017
Date Available: 27-Jul-2018
DDC Class: 540 Chemie und zugeordnete Wissenschaften
600 Technik, Technologie
Subject(s): glycopeptide antibiotics
nanoMIP
Gram-positive bacteria
Sponsor/Funder: DFG, TH 662/19-1, Open Access Publizieren 2017 - 2018 / Technische Universität Berlin
License: https://creativecommons.org/licenses/by/4.0/
Journal Title: Scientific Reports
Publisher: Nature Publishing Group
Publisher Place: London
Volume: 8
Article Number: 11222
Publisher DOI: 10.1038/s41598-018-29585-2
ISSN: 2045-2322
Appears in Collections:FG Technische Chemie » Publications

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