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Main Title: Comprehensive snake venomics of the Okinawa Habu pit viper, Protobothrops flavoviridis, by complementary mass spectrometry-guided approaches
Author(s): Damm, Maik
Hempel, Benjamin-Florian
Nalbantsoy, Ayse
Süßmuth, Roderich D.
Type: Article
Language Code: en
Abstract: The Asian world is home to a multitude of venomous and dangerous snakes, which are used to induce various medical effects in the preparation of traditional snake tinctures and alcoholics, like the Japanese snake wine, named Habushu. The aim of this work was to perform the first quantitative proteomic analysis of the Protobothrops flavoviridis pit viper venom. Accordingly, the venom was analyzed by complimentary bottom-up and top-down mass spectrometry techniques. The mass spectrometry-based snake venomics approach revealed that more than half of the venom is composed of different phospholipases A2 (PLA2). The combination of this approach and an intact mass profiling led to the identification of the three main Habu PLA2s. Furthermore, nearly one-third of the total venom consists of snake venom metalloproteinases and disintegrins, and several minor represented toxin families were detected: C-type lectin-like proteins (CTL), cysteine-rich secretory proteins (CRISP), snake venom serine proteases (svSP), l-amino acid oxidases (LAAO), phosphodiesterase (PDE) and 5′-nucleotidase. Finally, the venom of P. flavoviridis contains certain bradykinin-potentiating peptides and related peptides, like the svMP inhibitors, pEKW, pEQW, pEEW and pENW. In preliminary MTT cytotoxicity assays, the highest cancerous-cytotoxicity of crude venom was measured against human neuroblastoma SH-SY5Y cells and shows disintegrin-like effects in some fractions.
Issue Date: 29-Jul-2018
Date Available: 6-Aug-2018
DDC Class: 540 Chemie und zugeordnete Wissenschaften
Subject(s): snake venomics
Protobothrops flavoviridis
Habu pit viper
tripeptide metalloprotease inhibitor
Sponsor/Funder: DFG, TH 662/19-1, Open Access Publizieren 2017 - 2018 / Technische Universität Berlin
Journal Title: Molecules
Publisher: MDPI
Publisher Place: Basel
Volume: 23
Issue: 8
Article Number: 1893
Publisher DOI: 10.3390/molecules23081893
EISSN: 1420-3049
Appears in Collections:FG Biologische Chemie » Publications

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