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Main Title: Optimization of cell-laden bioinks for 3D bioprinting and efficient infection with influenza A virus
Author(s): Berg, Johanna
Hiller, Thomas
Kissner, Maya S.
Qazi, Taimoor H.
Duda, Georg N.
Hocke, Andreas C.
Hippenstiel, Stefan
Elomaa, Laura
Weinhart, Marie
Fahrenson, Christoph
Kurreck, Jens
Type: Article
Language Code: en
Abstract: Bioprinting is a new technology, which arranges cells with high spatial resolution, but its potential to create models for viral infection studies has not yet been fully realized. The present study describes the optimization of a bioink composition for extrusion printing. The bioinks were biophysically characterized by rheological and electron micrographic measurements. Hydrogels consisting of alginate, gelatin and Matrigel were used to provide a scaffold for a 3D arrangement of human alveolar A549 cells. A blend containing 20% Matrigel provided the optimal conditions for spatial distribution and viability of the printed cells. Infection of the 3D model with a seasonal influenza A strain resulted in widespread distribution of the virus and a clustered infection pattern that is also observed in the natural lung but not in two-dimensional (2D) cell culture, which demonstrates the advantage of 3D printed constructs over conventional culture conditions. The bioink supported viral replication and proinflammatory interferon release of the infected cells. We consider our strategy to be paradigmatic for the generation of humanized 3D tissue models by bioprinting to study infections and develop new antiviral strategies.
Issue Date: 17-Sep-2018
Date Available: 27-Sep-2018
DDC Class: 500 Naturwissenschaften und Mathematik
600 Technik, Technologie
Subject(s): bioprinting
influenza A virus
Sponsor/Funder: DFG, TH 662/19-1, Open Access Publizieren 2017 - 2018 / Technische Universität Berlin
Journal Title: Scientific Reports
Publisher: Nature Publishing Group
Publisher Place: London
Volume: 8
Article Number: 13877
Publisher DOI: 10.1038/s41598-018-31880-x
ISSN: 2045-2322
Appears in Collections:FG Angewandte Biochemie » Publications

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