Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-7387
Main Title: The Fatty Acid Synthase of the Basidiomycete Omphalotus olearius Is a Single Polypeptide
Author(s): Antelo, Luis
Schlipp, Angela
Hof, Carolin
Eisfeld, Katrin
Berg, Holger
Hornbogen, Till
Zocher, Rainer
Anke, Heidrun
Type: Article
Language Code: en
Abstract: Fatty acids are essential components of almost all biological membranes. Additionally, they are important in energy storage, as second messengers during signal transduction, and in post-translational protein modification. De novo synthesis of fatty acids is essential for almost all organisms, and entails the iterative elongation of the growing fatty acid chain through a set of reactions conserved in all kingdoms. During our work on the biosynthesis of secondary metabolites, a 450-kDa protein was detected by SDS-PAGE of enriched fractions from mycelial lysates from the basidiomycete Omphalotus olearius. Protein sequencing of this protein band revealed the presence of peptides with homology to both α and β subunits of the ascomycete fatty acid synthase (FAS) family. The FAS encoding gene of O. olearius was sequenced. The positions of its predicted 21 introns were verified. The gene encodes a 3931 amino acids single protein, with an equivalent of the ascomycetous β subunit at the N-terminus and the α subunit at the C-terminus. This is the first report on an FAS protein from a homobasidiomycete and also the first fungal FAS which is comprised of a single polypeptide.
URI: https://depositonce.tu-berlin.de//handle/11303/8236
http://dx.doi.org/10.14279/depositonce-7387
Issue Date: 2009
Date Available: 1-Oct-2018
DDC Class: 570 Biowissenschaften; Biologie
Subject(s): Omphalotus olearius
fatty acid synthase
Basidiomycete
License: https://creativecommons.org/licenses/by-nc-nd/3.0/
Journal Title: Zeitschrift für Naturforschung C
Publisher: De Gruyter
Publisher Place: Berlin
Volume: 64
Issue: 3-4
Publisher DOI: 10.1515/znc-2009-3-416
Page Start: 244
Page End: 250
EISSN: 1865-7125
ISSN: 0939-5075
Appears in Collections:Inst. Chemie » Publications

Files in This Item:
File Description SizeFormat 
antelo_et_al_2009.pdf200.21 kBAdobe PDFThumbnail
View/Open


This item is licensed under a Creative Commons License Creative Commons