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Main Title: Modeling of variant copies of subunit D1 in the structure of photosystem II from Thermosynechococcus elongatus
Author(s): Loll, Bernhard
Broser, Matthias
Kós, Peter B.
Kern, Jan
Biesiadka, Jacek
Vass, Imre
Saenger, Wolfram
Zouni, Athina
Type: Article
Language Code: en
Abstract: In the cyanobacterium Thermosynechococcus elongatus BP-1, living in hot springs, the light environment directly regulates expression of genes that encode key components of the photosynthetic multi-subunit protein-pigment complex photosystem II (PSII). Light is not only essential as an energy source to power photosynthesis, but leads to formation of aggressive radicals which induce severe damage of protein subunits and organic cofactors. Photosynthetic organisms develop several protection mechanisms against this photo-damage, such as the differential expression of genes coding for the reaction center subunit D1 in PSII. Testing the expression of the three different genes (psbAI, psbAII, psbAIII) coding for D1 in T. elongatus under culture conditions used for preparing the material used in crystallization of PSII showed that under these conditions only subunit PsbA1 is present. However, exposure to high-light intensity induced partial replacement of PsbA1 with PsbA3. Modeling of the variant amino acids of the three different D1 copies in the 3.0 Å resolution crystal structure of PSII revealed that most of them are in the direct vicinity to redox-active cofactors of the electron transfer chain. Possible structural and mechanistic consequences for electron transfer are discussed.
Issue Date: 2008
Date Available: 1-Oct-2018
DDC Class: 540 Chemie und zugeordnete Wissenschaften
570 Biowissenschaften; Biologie
Subject(s): gene regulation
light adaptation
protein-cofactor interaction
Sponsor/Funder: DFG, SFB 498, Protein-Kofaktor-Wechselwirkungen in biologischen Prozessen
EC/FP6/516510/EU/Linking molecular genetics and bio-mimetic chemistry - a multidisciplinary approach to achieve renewable hydrogen production/SOLAR-H
Journal Title: Biological chemistry
Publisher: De Gruyter
Publisher Place: Berlin
Volume: 389
Issue: 5
Publisher DOI: 10.1515/BC.2008.058
Page Start: 609
Page End: 617
EISSN: 1437-4315
ISSN: 1431-6730
Notes: Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
Appears in Collections:Inst. Chemie » Publications

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