Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-7621
Main Title: Crystallization of Intact and Subunit L-Deficient Monomers from Synechocystis PCC 6803 Photosystem I
Author(s): Jekow, Petra
Schubert, Wolf-Dieter
Fromme, Petra
Kruip, Jochen
Chitnis, Parag R.
Rögner, Matthias
Saenger, Wolfram
Type: Article
Language Code: en
Abstract: Photosystem I monomers from wildtype cells of Synechocystis PCC 6803 and from a mu­tant deficient in the psaL gene were crystallized. PsaL encodes for the hydrophobic subunit L, which has been proposed to constitute the trimerization domain in the PS I trimer. The absence of subunit L facilitated crystallization of the PS I monomer. The unit cell dimensions and the space group for the crystals from this preparation could be determined to be a = b = 132 Å , c -525 Å, α = β = 90°, y = 120°, the space group is P61 or P65. The results show the potential of using specifically designed deletion mutants of an integral membrane protein for the systematic improvement of crystal structure data.
URI: https://depositonce.tu-berlin.de//handle/11303/8475
http://dx.doi.org/10.14279/depositonce-7621
Issue Date: 1996
Date Available: 10-Nov-2018
DDC Class: 570 Biowissenschaften; Biologie
Subject(s): Photosystem I
PS I monomers
crystallization
mesophilic cyanobacterium
License: https://creativecommons.org/licenses/by-nc-nd/3.0/
Journal Title: Zeitschrift für Naturforschung C
Publisher: De Gruyter
Publisher Place: Berlin
Volume: 51
Issue: 3-4
Publisher DOI: 10.1515/znc-1996-3-410
Page Start: 195
Page End: 199
EISSN: 1865-7125
ISSN: 0939-5075
Appears in Collections:Inst. Chemie » Publications

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