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Main Title: Robotic Platform for Parallelized Cultivation and Monitoring of Microbial Growth Parameters in Microwell Plates
Author(s): Knepper, Andreas
Heiser, Michael
Glauche, Florian
Neubauer, Peter
Type: Article
Language Code: en
Abstract: The enormous variation possibilities of bioprocesses challenge process development to fix a commercial process with respect to costs and time. Although some cultivation systems and some devices for unit operations combine the latest technology on miniaturization, parallelization, and sensing, the degree of automation in upstream and downstream bioprocess development is still limited to single steps. We aim to face this challenge by an interdisciplinary approach to significantly shorten development times and costs. As a first step, we scaled down analytical assays to the microliter scale and created automated procedures for starting the cultivation and monitoring the optical density (OD), pH, concentrations of glucose and acetate in the culture medium, and product formation in fed-batch cultures in the 96-well format. Then, the separate measurements of pH, OD, and concentrations of acetate and glucose were combined to one method. This method enables automated process monitoring at dedicated intervals (e.g., also during the night). By this approach, we managed to increase the information content of cultivations in 96-microwell plates, thus turning them into a suitable tool for high-throughput bioprocess development. Here, we present the flowcharts as well as cultivation data of our automation approach.
Issue Date: 2014
Date Available: 8-Jan-2019
DDC Class: 620 Ingenieurwissenschaften und zugeordnete Tätigkeiten
Subject(s): laboratory automation
consistent bioprocess development
high throughput
microliter scale
Journal Title: Journal of Laboratory Automation
Publisher: SAGE Publications
Publisher Place: Washington, DC
Volume: 19
Issue: 6
Publisher DOI: 10.1177/2211068214547231
Page Start: 593
Page End: 601
EISSN: 1540-2452
ISSN: 2211-0682
Notes: Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
Appears in Collections:FG Bioverfahrenstechnik » Publications

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