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Main Title: Application of a Rapid and Integrated Analysis System (RIAS) as a High-Throughput Processing Tool for In Vitro ADME Samples by Liquid Chromatography/Tandem Mass Spectrometry
Author(s): Luippold, Andreas H.
Arnhold, Thomas
Jörg, Wolfgang
Krüger, Beate
Süßmuth, Roderich D.
Type: Article
Language Code: en
Abstract: Over the past decade, drug discovery programs have started to address the optimization of key ADME properties already at an early stage of the process. Hence, analytical chemists have been confronted with tremendously rising sample numbers and have had to develop methodologies accelerating quantitative liquid chromatography/tandem mass spectrometry (LC/MS/MS). This article focuses on the application of a generic and fully automated LC/MS/MS, named Rapid and Integrated Analysis System (RIAS), as a high-throughput platform for the rapid quantification of drug-like compounds in various in vitro ADME assays. Previous efforts were dedicated to the setup and feasibility study of a workflow-integrated platform combining a modified high-throughput liquid handling LC/MS/MS system controlled by a customized software interface and a customized data-processing and reporting tool. Herein the authors present an extension of this previously developed basic application to a broad set of ADME screening campaigns, covering CYP inhibition, Caco-2, and PAMPA assays. The platform is capable of switching automatically between various ADME assays, performs MS compound optimization if required, and provides a speed of 8 s from sample to sample, independently of the type of ADME assay. Quantification and peak review are adopted to the high-throughput environment and tested against a standard HPLC-ESI technology.
Issue Date: 2011
Date Available: 8-Jan-2019
DDC Class: 570 Biowissenschaften; Biologie
Subject(s): high-throughput
in vitro ADME
active barcoding
Journal Title: Journal of Biomolecular Screening
Publisher: SAGE Publications
Publisher Place: Washington, DC
Volume: 16
Issue: 3
Publisher DOI: 10.1177/1087057110397358
Page Start: 370
Page End: 377
EISSN: 1552-454X
ISSN: 1087-0571
Notes: Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
Appears in Collections:FG Biologische Chemie » Publications

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