FG Bioenergetik

7 Items

Recent Submissions
Design and utilization of NADH sensors in bacterial cells, specifically to monitor activity of the soluble hydrogenase of Ralstonia eutropha

Wilkening, Svea Kristina (2021)

The soluble hydrogenase (SH) of R. eutropha is a potential candidate for application in clean energy generation in a post-fossil age. In order to elucidate its mode of action, and possibly deduce general parameters for a biotechnological implementation, it is desirable to monitor the protein in vivo. The hydrogenase couples the oxidation of H2 to the reduction of NAD+ to NADH, offering the poss...

An unusual role for the phytyl chains in the photoprotection of the chlorophylls bound to Water-Soluble Chlorophyll-binding Proteins

Agostini, Alessandro ; Palm, Daniel M. ; Schmitt, Franz-Josef ; Albertini, Marco ; Valentin, Marilena Di ; Paulsen, Harald ; Carbonera, Donatella (2017-08-07)

Water-Soluble Chlorophyll Proteins (WSCPs) from Brassicaceae are non-photosynthetic proteins which tetramerize upon binding four chlorophyll (Chl) molecules. The bound Chls are highly photostable, despite the lack of bound carotenoids known, in Chl-containing photosynthetic proteins, to act as singlet oxygen and Chl triplet (3Chl) quenchers. Although the physiological function of WSCPs is still...

Soluble Cyanobacterial Carotenoprotein as a Robust Antioxidant Nanocarrier and Delivery Module

Maksimov, Eugene G. ; Zamaraev, Alexey V. ; Parshina, Evgenia Yu. ; Slonimskiy, Yury B. ; Slastnikova, Tatiana A. ; Abdrakhmanov, Alibek A. ; Babaev, Pavel A. ; Efimova, Svetlana S. ; Ostroumova, Olga S. ; Stepanov, Alexey V. ; Slutskaya, Ekaterina A. ; Ryabova, Anastasia V. ; Friedrich, Thomas ; Sluchanko, Nikolai N. (2020-09-15)

To counteract oxidative stress, antioxidants including carotenoids are highly promising, yet their exploitation is drastically limited by the poor bioavailability and fast photodestruction, whereas current delivery systems are far from being efficient. Here we demonstrate that the recently discovered nanometer-sized water-soluble carotenoprotein from Anabaena sp. PCC 7120 (termed AnaCTDH) trans...

Voltage sensitive phosphatases: emerging kinship to protein tyrosine phosphatases from structure-function research

Hobiger, Kirstin ; Friedrich, Thomas (2015-01-10)

The transmembrane protein Ci-VSP from the ascidian Ciona intestinalis was described as first member of a fascinating family of enzymes, the voltage sensitive phosphatases (VSPs). Ci-VSP and its voltage-activated homologs from other species are stimulated by positive membrane potentials and dephosphorylate the head groups of negatively charged phosphoinositide phosphates (PIPs). In doing so, VSP...

ATP1A2 Mutations in Migraine: Seeing through the Facets of an Ion Pump onto the Neurobiology of Disease

Friedrich, Thomas ; Tavraz, Neslihan N. ; Junghans, Cornelia (2016-06-21)

Mutations in four genes have been identified in familial hemiplegic migraine (FHM), from which CACNA1A (FHM type 1) and SCN1A (FHM type 3) code for neuronal voltage-gated calcium or sodium channels, respectively, while ATP1A2 (FHM type 2) encodes the α2 isoform of the Na+,K+-ATPase's catalytic subunit, thus classifying FHM primarily as an ion channel/ion transporter pathology. FHM type 4 is att...

The role of local and remote amino acid substitutions for optimizing fluorescence in bacteriophytochromes: A case study on iRFP

Buhrke, David ; Vélazquez Escobar, Francisco ; Sauthof, Luisa ; Wilkening, Svea ; Herder, Nico ; Tavraz, Neslihan N. ; Willoweit, Mario ; Keidel, Anke ; Utesch, Tillmann ; Mroginski, Maria-Andrea ; Schmitt, Franz-Josef ; Hildebrandt, Peter ; Friedrich, Thomas (2016-06-22)

Bacteriophytochromes are promising tools for tissue microscopy and imaging due to their fluorescence in the near-infrared region. These applications require optimization of the originally low fluorescence quantum yields via genetic engineering. Factors that favour fluorescence over other non-radiative excited state decay channels are yet poorly understood. In this work we employed resonance Ram...

Chromophore binding to two cysteines increases quantum yield of near-infrared fluorescent proteins

Buhrke, David ; Tavraz, Neslihan N. ; Shcherbakova, Daria M. ; Sauthof, Luisa ; Moldenhauer, Marcus ; Vélazquez Escobar, Francisco ; Verkhusha, Vladislav V. ; Hildebrandt, Peter ; Friedrich, Thomas (2019-02-12)

Phytochromes are red/far-red light sensing photoreceptors employing linear tetrapyrroles as chromophores, which are covalently bound to a cysteine (Cys) residue in the chromophore-binding domain (CBD, composed of a PAS and a GAF domain). Recently, near-infrared (NIR) fluorescent proteins (FPs) engineered from bacterial phytochromes binding biliverdin IXα (BV), such as the iRFP series, have beco...