Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-8730
Full metadata record
DC FieldValueLanguage
dc.contributor.authorMasdor, Noor Azlina-
dc.contributor.authorAltintas, Zeynep-
dc.contributor.authorTothill, Ibtisam E.-
dc.date.accessioned2019-08-02T07:35:24Z-
dc.date.available2019-08-02T07:35:24Z-
dc.date.issued2017-05-16-
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/9690-
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-8730-
dc.description.abstractCampylobacteriosis is an internationally important foodborne disease caused by Campylobacter jejuni. The bacterium is prevalent in chicken meat and it is estimated that as much as 90% of chicken meat on the market may be contaminated with the bacterium. The current gold standard for the detection of C. jejuni is the culturing method, which takes at least 48 h to confirm the presence of the bacterium. Hence, the aim of this work was to investigate the development of a Surface Plasmon Resonance (SPR) sensor platform for C. jejuni detection. Bacterial strains were cultivated in-house and used in the development of the sensor. SPR sensor chips were first functionalized with polyclonal antibodies raised against C. jejuni using covalent attachment. The gold chips were then applied for the direct detection of C. jejuni. The assay conditions were then optimized and the sensor used for C. jejuni detection, achieving a detection limit of 8 × 106 CFU·mL−1. The sensitivity of the assay was further enhanced to 4 × 104 CFU·mL−1 through the deployment of a sandwich assay format using the same polyclonal antibody. The LOD obtained in the sandwich assay was higher than that achieved using commercial enzyme-linked immunosorbent assay (ELISA) (106–107 CFU·mL−1). This indicate that the SPR-based sandwich sensor method has an excellent potential to replace ELISA tests for C. jejuni detection. Specificity studies performed with Gram-positive and Gram-negative bacteria, demonstrated the high specific of the sensor for C. jejuni.en
dc.language.isoenen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subject.ddc540 Chemie und zugeordnete Wissenschaftende
dc.subject.otherCampylobacter jejunien
dc.subject.othercampylobacteriosisen
dc.subject.otherSurface Plasmon Resonanceen
dc.subject.otherimmunosensoren
dc.subject.othersandwich assayen
dc.subject.otherSPRen
dc.titleSurface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejunien
dc.typeArticleen
dc.date.updated2019-07-31T12:23:02Z-
tub.accessrights.dnbfreeen
tub.publisher.universityorinstitutionTechnische Universität Berlinen
dc.identifier.eissn2227-9040-
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.doi10.3390/chemosensors5020016en
dcterms.bibliographicCitation.journaltitleChemosensorsen
dcterms.bibliographicCitation.originalpublisherplaceBaselen
dcterms.bibliographicCitation.volume5en
dcterms.bibliographicCitation.originalpublishernameMDPIen
dcterms.bibliographicCitation.issue2en
dcterms.bibliographicCitation.articlenumber16en
Appears in Collections:FG Technische Chemie » Publications

Files in This Item:
File Description SizeFormat 
chemosensors-05-00016.pdf3.03 MBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons