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Main Title: Reproduction of Large-Scale Bioreactor Conditions on Microfluidic Chips
Author(s): Ho, Phuong
Westerwalbesloh, Christoph
Kaganovitch, Eugen
Grünberger, Alexander
Neubauer, Peter
Kohlheyer, Dietrich
Lieres, Eric von
Type: Article
Language Code: en
Abstract: Microbial cells in industrial large-scale bioreactors are exposed to fluctuating conditions, e.g., nutrient concentration, dissolved oxygen, temperature, and pH. These inhomogeneities can influence the cell physiology and metabolism, e.g., decelerate cell growth and product formation. Microfluidic systems offer new opportunities to study such effects in great detail by examining responses to varying environmental conditions at single-cell level. However, the possibility to reproduce large-scale bioreactor conditions in microscale cultivation systems has not yet been systematically investigated. Hence, we apply computational fluid dynamics (CFD) simulations to analyze and compare three commonly used microfluidic single-cell trapping and cultivation devices that are based on (i) mother machines (MM), (ii) monolayer growth chambers (MGC), and (iii) negative dielectrophoresis (nDEP). Several representative time-variant nutrient concentration profiles are applied at the chip entry. Responses to these input signals within the studied microfluidic devices are comparatively evaluated at the positions of the cultivated cells. The results are comprehensively presented in a Bode diagram that illustrates the degree of signal damping depending on the frequency of change in the inlet concentration. As a key finding, the MM can accurately reproduce signal changes that occur within 1 s or slower, which are typical for the environmental conditions observed by single cells in large-scale bioreactors, while faster changes are levelled out. In contrast, the nDEP and MGC are found to level out signal changes occurring within 10 s or faster, which can be critical for the proposed application.
Issue Date: 19-Apr-2019
Date Available: 12-Aug-2019
DDC Class: 600 Technik, Technologie
570 Biowissenschaften; Biologie
Subject(s): microfluidics
single-cell analysis
computational fluid dynamics
frequency response
life line
monolayer growth chamber
mother machine
negative dielectrophoresis
Sponsor/Funder: BMBF, 031A302C, e:Bio - Modul II - Verbundprojekt: 0,6Plus - Verbesserung grundlegender Wachstumseigenschaften von C. glutamicum zur Verbreiterung der industriellen Anwendbarkeit - Teilprojekt C
BMBF, 031A095A, Biotechnologie2020+ Strukturvorhaben: Helmholtz Research Network - Molecular Interaction Engineering; Teilprojekt des FZJ
Journal Title: Microorganisms
Publisher: MDPI
Publisher Place: Basel
Volume: 7
Issue: 4
Article Number: 105
Publisher DOI: 10.3390/microorganisms7040105
EISSN: 2076-2607
Appears in Collections:FG Bioverfahrenstechnik » Publications

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