Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-8836
Main Title: Comparison of Raman and Mid-Infrared Spectroscopy for Real-Time Monitoring of Yeast Fermentations: A Proof-of-Concept for Multi-Channel Photometric Sensors
Author(s): Schalk, Robert
Heintz, Annabell
Braun, Frank
Iacono, Giuseppe
Rädle, Matthias
Gretz, Norbert
Methner, Frank-Jürgen
Beuermann, Thomas
Type: Article
Language Code: en
Abstract: Raman and mid-infrared (MIR) spectroscopy are useful tools for the specific detection of molecules, since both methods are based on the excitation of fundamental vibration modes. In this study, Raman and MIR spectroscopy were applied simultaneously during aerobic yeast fermentations of Saccharomyces cerevisiae. Based on the recorded Raman intensities and MIR absorption spectra, respectively, temporal concentration courses of glucose, ethanol, and biomass were determined. The chemometric methods used to evaluate the analyte concentrations were partial least squares (PLS) regression and multiple linear regression (MLR). In view of potential photometric sensors, MLR models based on two (2D) and four (4D) analyte-specific optical channels were developed. All chemometric models were tested to predict glucose concentrations between 0 and 30 g L−1, ethanol concentrations between 0 and 10 g L−1, and biomass concentrations up to 15 g L−1 in real time during diauxic growth. Root-mean-squared errors of prediction (RMSEP) of 0.68 g L−1, 0.48 g L−1, and 0.37 g L−1 for glucose, ethanol, and biomass were achieved using the MIR setup combined with a PLS model. In the case of Raman spectroscopy, the corresponding RMSEP values were 0.92 g L−1, 0.39 g L−1, and 0.29 g L−1. Nevertheless, the simple 4D MLR models could reach the performance of the more complex PLS evaluation. Consequently, the replacement of spectrometer setups by four-channel sensors were discussed. Moreover, the advantages and disadvantages of Raman and MIR setups are demonstrated with regard to process implementation.
URI: https://depositonce.tu-berlin.de/handle/11303/9815
http://dx.doi.org/10.14279/depositonce-8836
Issue Date: 17-Jun-2019
Date Available: 15-Aug-2019
DDC Class: 660 Chemische Verfahrenstechnik
Subject(s): Raman spectroscopy
mid-infrared spectroscopy
fermentation of Saccharomyces cerevisiae
real-time monitoring
multi-channel photometric sensors
multiple linear regression
partial least squares regression
monitoring of glucose
ethanol
biomass
License: https://creativecommons.org/licenses/by/4.0/
Journal Title: Applied Sciences
Publisher: MDPI
Publisher Place: Basel
Volume: 9
Issue: 12
Article Number: 2472
Publisher DOI: 10.3390/app9122472
EISSN: 2076-3417
Appears in Collections:FG Brauwesen » Publications

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