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Main Title: Moulding the mould: understanding and reprogramming filamentous fungal growth and morphogenesis for next generation cell factories
Author(s): Cairns, Timothy C.
Zheng, Xiaomei
Zheng, Ping
Sun, Jibin
Meyer, Vera
Type: Article
Language Code: en
Abstract: Filamentous fungi are harnessed as cell factories for the production of a diverse range of organic acids, proteins, and secondary metabolites. Growth and morphology have critical implications for product titres in both submerged and solid-state fermentations. Recent advances in systems-level understanding of the filamentous lifestyle and development of sophisticated synthetic biological tools for controlled manipulation of fungal genomes now allow rational strain development programs based on data-driven decision making. In this review, we focus on Aspergillus spp. and other industrially utilised fungi to summarise recent insights into the multifaceted and dynamic relationship between filamentous growth and product titres from genetic, metabolic, modelling, subcellular, macromorphological and process engineering perspectives. Current progress and knowledge gaps with regard to mechanistic understanding of product secretion and export from the fungal cell are discussed. We highlight possible strategies for unlocking lead genes for rational strain optimizations based on omics data, and discuss how targeted genetic manipulation of these candidates can be used to optimise fungal morphology for improved performance. Additionally, fungal signalling cascades are introduced as critical processes that can be genetically targeted to control growth and morphology during biotechnological applications. Finally, we review progress in the field of synthetic biology towards chassis cells and minimal genomes, which will eventually enable highly programmable filamentous growth and diversified production capabilities. Ultimately, these advances will not only expand the fungal biotechnology portfolio but will also significantly contribute to a sustainable bio-economy.
Issue Date: 2-Apr-2019
Date Available: 22-Aug-2019
DDC Class: 570 Biowissenschaften; Biologie
660 Chemische Verfahrenstechnik
Subject(s): cell factory
filamentous fungi
aspergillus niger
systems biology
synthetic biology
protein secretion
secondary metabolite
citric acid
Sponsor/Funder: TU Berlin, Open-Access-Mittel - 2019
Journal Title: Biotechnology for biofuels
Publisher: BioMed Central
Publisher Place: London
Volume: 12
Issue: 1
Article Number: 77
Publisher DOI: 10.1186/s13068-019-1400-4
EISSN: 1754-6834
Appears in Collections:FG Angewandte und Molekulare Mikrobiologie » Publications

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