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Main Title: Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications
Author(s): Schimek, Katharina
Hsu, Hao-Hsiang
Boehme, Moritz
Kornet, Jacob Jan
Marx, Uwe
Lauster, Roland
Pörtner, Ralf
Lindner, Gerd
Type: Article
Language Code: en
Abstract: The human skin is involved in protecting the inner body from constant exposure to outer environmental stimuli. There is an evident need to screen for toxicity and the efficacy of drugs and cosmetics applied to the skin. To date, animal studies are still the standard method for substance testing, although they are currently controversially discussed Therefore, the multi-organ chip is an attractive alternative to replace animal testing. The two-organ chip is designed to hold 96-well cell culture inserts (CCIs). Small-sized skin equivalents are needed for this. In this study, full-thickness skin equivalents (ftSEs) were generated successfully inside 96-well CCIs. These skin equivalents developed with in vivo-like histological architecture, with normal differentiation marker expressions and proliferation rates. The 96-well CCI-based ftSEs were successfully integrated into the two-organ chip. The permeation of fluorescein sodium salt through the ftSEs was monitored during the culture. The results show a decreasing value for the permeation over time, which seems a promising method to track the development of the ftSEs. Additionally, the permeation was implemented in a computational fluid dynamics simulation, as a tool to predict results in long-term experiments. The advantage of these ftSEs is the reduced need for cells and substances, which makes them more suitable for high throughput assays.
Issue Date: 7-Jun-2018
Date Available: 30-Aug-2019
DDC Class: 570 Biowissenschaften; Biologie
Subject(s): full thickness skin equivalents
multi-organ chip
substance permeation
96-well cell culture insert
Journal Title: Bioengineering
Publisher: MDPI
Publisher Place: Basel
Volume: 5
Issue: 2
Article Number: 43
Publisher DOI: 10.3390/bioengineering5020043
EISSN: 2306-5354
Appears in Collections:FG Medizinische Biotechnologie » Publications

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