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Main Title: Toxicity Assay for Citrinin, Zearalenone and Zearalenone-14-Sulfate Using the Nematode Caenorhabditis elegans as Model Organism
Author(s): Keller, Julia
Borzekowski, Antje
Haase, Hajo
Menzel, Ralph
Rueß, Liliane
Koch, Matthias
Type: Article
Language Code: en
Abstract: To keep pace with the rising number of detected mycotoxins, there is a growing need for fast and reliable toxicity tests to assess potential threats to food safety. Toxicity tests with the bacterial-feeding nematode Caenorhabditis elegans as the model organism are well established. In this study the C. elegans wildtype strain N2 (var. Bristol) was used to investigate the toxic effects of the food-relevant mycotoxins citrinin (CIT) and zearalenone-14-sulfate (ZEA-14-S) and zearalenone (ZEA) on different life cycle parameters including reproduction, thermal and oxidative stress resistance and lifespan. The metabolization of the mycotoxins by the nematodes in vivo was investigated using HPLC-MS/MS. ZEA was metabolized in vivo to the reduced isomers α-zearalenol (α-ZEL) and β-ZEL. ZEA-14-S was reduced to α-/β-ZEL-14-sulfate and CIT was metabolized to mono-hydroxylated CIT. All mycotoxins tested led to a significant decrease in the number of nematode offspring produced. ZEA and CIT displayed negative effects on stress tolerance levels and for CIT an additional shortening of the mean lifespan was observed. In the case of ZEA-14-S, however, the mean lifespan was prolonged. The presented study shows the applicability of C. elegans for toxicity testing of emerging food mycotoxins for the purpose of assigning potential health threats.
Issue Date: 9-Jul-2018
Date Available: 10-Sep-2019
DDC Class: 610 Medizin und Gesundheit
Subject(s): mycotoxin
toxicity testing
food safety
Caenorhabditis elegans
Journal Title: Toxins
Publisher: MDPI
Publisher Place: Basel
Volume: 10
Issue: 7
Article Number: 284
Publisher DOI: 10.3390/toxins10070284
EISSN: 2072-6651
Appears in Collections:FG Lebensmittelchemie und Toxikologie » Publications

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