Detection of Listeria innocua on roll-to-roll produced SERS substrates with gold nanoparticles

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dc.contributor.authorUusitalo, S.
dc.contributor.authorKögler, Martin
dc.contributor.authorVälimaa, A.-L.
dc.contributor.authorPopov, A.
dc.contributor.authorRyabchikov, Yu.
dc.contributor.authorKontturi, V.
dc.contributor.authorSiitonen, S.
dc.contributor.authorPetäjä, J.
dc.contributor.authorVirtanen, T.
dc.contributor.authorLaitinen, R.
dc.contributor.authorKinnunen, M.
dc.contributor.authorMeglinski, I.
dc.contributor.authorKabashin, A.
dc.contributor.authorBunker, A.
dc.contributor.authorViitala, T.
dc.contributor.authorHiltunen, J.
dc.date.accessioned2018-04-19T08:47:44Z
dc.date.available2018-04-19T08:47:44Z
dc.date.issued2016
dc.description.abstractThe rapid and accurate detection of food pathogens plays a critical role in the early prevention of foodborne epidemics. Current bacteria identification practices, including colony counting, polymerase chain reaction (PCR) and immunological methods, are time consuming and labour intensive; they are not ideal for achieving the required immediate diagnosis. Different SERS substrates have been studied for the detection of foodborne microbes. The majority of the approaches are either based on costly patterning techniques on silicon or glass wafers or on methods which have not been tested in large scale fabrication. We demonstrate the feasibility of analyte specific sensing using mass-produced, polymer-based low-cost SERS substrate in analysing the chosen model microbe with biological recognition. The use of this novel roll-to-roll fabricated SERS substrate was combined with optimised gold nanoparticles to increase the detection sensitivity. Distinctive SERS spectral bands were recorded for Listeria innocua ATCC 33090 using an in-house build (785 nm) near infra red (NIR) Raman system. Results were compared to both those found in the literature and the results obtained from a commercial time-gated Raman system with a 532 nm wavelength laser excitation. The effect of the SERS enhancer metal and the excitation wavelength on the detected spectra was found to be negligible. The hypothesis that disagreements within the literature regarding bacterial spectra results from conditions present during the detection process has not been supported. The sensitivity of our SERS detection was improved through optimization of the concentration of the sample inside the hydrophobic polydimethylsiloxane (PDMS) wells. Immunomagnetic separation (IMS) beads were used to assist the accumulation of bacteria into the path of the beam of the excitation laser. With this combination we have detected Listeria with gold enhanced SERS in a label free manner from such low sample concentrations as 104 CFU ml−1.en
dc.identifier.eissn2046-2069
dc.identifier.issn2046-2069
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/7644
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-6834
dc.language.isoen
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.subject.ddc540 Chemie und zugeordnete Wissenschaften
dc.titleDetection of Listeria innocua on roll-to-roll produced SERS substrates with gold nanoparticlesen
dc.typeArticle
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.doi10.1039/c6ra08313g
dcterms.bibliographicCitation.issue67
dcterms.bibliographicCitation.journaltitleRSC Advancesen
dcterms.bibliographicCitation.originalpublishernameRoyal Society of Chemistry (RSC)
dcterms.bibliographicCitation.originalpublisherplaceLondon
dcterms.bibliographicCitation.pageend62989
dcterms.bibliographicCitation.pagestart62981
dcterms.bibliographicCitation.volume6
tub.accessrights.dnbfree
tub.affiliationFak. 3 Prozesswissenschaften::Inst. Biotechnologie::FG Bioverfahrenstechnikde
tub.affiliation.facultyFak. 3 Prozesswissenschaftende
tub.affiliation.groupFG Bioverfahrenstechnikde
tub.affiliation.instituteInst. Biotechnologiede
tub.publisher.universityorinstitutionTechnische Universität Berlinde

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