dc.contributor.author	Ramm, Franziska
dc.contributor.author	Dondapati, Srujan K.
dc.contributor.author	Trinh, Hoai Anh
dc.contributor.author	Wenzel, Dana
dc.contributor.author	Walter, Ruben M.
dc.contributor.author	Zemella, Anne
dc.contributor.author	Kubick, Stefan
dc.date.accessioned	2022-05-10T09:55:53Z
dc.date.available	2022-05-10T09:55:53Z
dc.date.issued	2022-04-19
dc.date.updated	2022-05-05T07:02:15Z
dc.description.abstract	The ongoing pandemic caused by the novel coronavirus (SARS-CoV-2) has led to more than 445 million infections and the underlying disease, COVID-19, resulted in more than 6 million deaths worldwide. The scientific world is already predicting future zoonotic diseases. Hence, rapid response systems are needed to tackle future epidemics and pandemics. Here, we present the use of eukaryotic cell-free systems for the rapid response to novel zoonotic diseases represented by SARS-CoV-2. Non-structural, structural and accessory proteins encoded by SARS-CoV-2 were synthesized by cell-free protein synthesis in a fast and efficient manner. The inhibitory effect of the non-structural protein 1 on protein synthesis could be shown in vitro. Structural proteins were quantitatively detected by commercial antibodies, therefore facilitating cell-free systems for the validation of available antibodies. The cytotoxic envelope protein was characterized in electrophysiological planar lipid bilayer measurements. Hence, our study demonstrates the potential of eukaryotic cell-free systems as a rapid response mechanism for the synthesis, functional characterization and antibody validation against a viral pathogen.	en
dc.description.sponsorship	BMBF, L1FHG42421, Unterstützung anwendungsorientierte Forschung für Fraunhofer 2021	en
dc.identifier.eissn	2296-4185
dc.identifier.uri	https://depositonce.tu-berlin.de/handle/11303/16835
dc.identifier.uri	http://dx.doi.org/10.14279/depositonce-15613
dc.language.iso	en	en
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	en
dc.subject.ddc	570 Biowissenschaften; Biologie	de
dc.subject.other	eukaryotic cell-free systems	en
dc.subject.other	SARS-CoV-2	en
dc.subject.other	viral proteins	en
dc.subject.other	rapid response	en
dc.subject.other	protein analytics	en
dc.subject.other	planar lipid bilayer measurements	en
dc.title	The Potential of Eukaryotic Cell-Free Systems as a Rapid Response to Novel Zoonotic Pathogens: Analysis of SARS-CoV-2 Viral Proteins	en
dc.type	Article	en
dc.type.version	publishedVersion	en
dcterms.bibliographicCitation.articlenumber	896751	en
dcterms.bibliographicCitation.doi	10.3389/fbioe.2022.896751	en
dcterms.bibliographicCitation.journaltitle	Frontiers in Bioengineering and Biotechnology	en
dcterms.bibliographicCitation.originalpublishername	Frontiers	en
dcterms.bibliographicCitation.originalpublisherplace	Lausanne	en
dcterms.bibliographicCitation.volume	10	en
tub.accessrights.dnb	free	en
tub.affiliation	Fak. 3 Prozesswissenschaften::Inst. Biotechnologie::FG Angewandte Biochemie	de
tub.affiliation.faculty	Fak. 3 Prozesswissenschaften	de
tub.affiliation.group	FG Angewandte Biochemie	de
tub.affiliation.institute	Inst. Biotechnologie	de
tub.publisher.universityorinstitution	Technische Universität Berlin	en

The Potential of Eukaryotic Cell-Free Systems as a Rapid Response to Novel Zoonotic Pathogens: Analysis of SARS-CoV-2 Viral Proteins

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