The Potential of Eukaryotic Cell-Free Systems as a Rapid Response to Novel Zoonotic Pathogens: Analysis of SARS-CoV-2 Viral Proteins

dc.contributor.authorRamm, Franziska
dc.contributor.authorDondapati, Srujan K.
dc.contributor.authorTrinh, Hoai Anh
dc.contributor.authorWenzel, Dana
dc.contributor.authorWalter, Ruben M.
dc.contributor.authorZemella, Anne
dc.contributor.authorKubick, Stefan
dc.date.accessioned2022-05-10T09:55:53Z
dc.date.available2022-05-10T09:55:53Z
dc.date.issued2022-04-19
dc.date.updated2022-05-05T07:02:15Z
dc.description.abstractThe ongoing pandemic caused by the novel coronavirus (SARS-CoV-2) has led to more than 445 million infections and the underlying disease, COVID-19, resulted in more than 6 million deaths worldwide. The scientific world is already predicting future zoonotic diseases. Hence, rapid response systems are needed to tackle future epidemics and pandemics. Here, we present the use of eukaryotic cell-free systems for the rapid response to novel zoonotic diseases represented by SARS-CoV-2. Non-structural, structural and accessory proteins encoded by SARS-CoV-2 were synthesized by cell-free protein synthesis in a fast and efficient manner. The inhibitory effect of the non-structural protein 1 on protein synthesis could be shown in vitro. Structural proteins were quantitatively detected by commercial antibodies, therefore facilitating cell-free systems for the validation of available antibodies. The cytotoxic envelope protein was characterized in electrophysiological planar lipid bilayer measurements. Hence, our study demonstrates the potential of eukaryotic cell-free systems as a rapid response mechanism for the synthesis, functional characterization and antibody validation against a viral pathogen.en
dc.description.sponsorshipBMBF, L1FHG42421, Unterstützung anwendungsorientierte Forschung für Fraunhofer 2021en
dc.identifier.eissn2296-4185
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/16835
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-15613
dc.language.isoenen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subject.ddc570 Biowissenschaften; Biologiede
dc.subject.othereukaryotic cell-free systemsen
dc.subject.otherSARS-CoV-2en
dc.subject.otherviral proteinsen
dc.subject.otherrapid responseen
dc.subject.otherprotein analyticsen
dc.subject.otherplanar lipid bilayer measurementsen
dc.titleThe Potential of Eukaryotic Cell-Free Systems as a Rapid Response to Novel Zoonotic Pathogens: Analysis of SARS-CoV-2 Viral Proteinsen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.articlenumber896751en
dcterms.bibliographicCitation.doi10.3389/fbioe.2022.896751en
dcterms.bibliographicCitation.journaltitleFrontiers in Bioengineering and Biotechnologyen
dcterms.bibliographicCitation.originalpublishernameFrontiersen
dcterms.bibliographicCitation.originalpublisherplaceLausanneen
dcterms.bibliographicCitation.volume10en
tub.accessrights.dnbfreeen
tub.affiliationFak. 3 Prozesswissenschaften>Inst. Biotechnologie>FG Angewandte Biochemiede
tub.affiliation.facultyFak. 3 Prozesswissenschaftende
tub.affiliation.groupFG Angewandte Biochemiede
tub.affiliation.instituteInst. Biotechnologiede
tub.publisher.universityorinstitutionTechnische Universität Berlinen
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