Combined Molecular and Elemental Mass Spectrometry Approaches for Absolute Quantification of Proteomes: Application to the Venomics Characterization of the Two Species of Desert Black Cobras, Walterinnesia aegyptia and Walterinnesia morgani

dc.contributor.authorCalvete, Juan J.
dc.contributor.authorPla, Davinia
dc.contributor.authorEls, Johannes
dc.contributor.authorCarranza, Salvador
dc.contributor.authorDamm, Maik
dc.contributor.authorHempel, Benjamin-Florian
dc.contributor.authorJohn, Elisa B. O.
dc.contributor.authorPetras, Daniel
dc.contributor.authorHeiss, Paul
dc.contributor.authorNalbantsoy, Ayse
dc.contributor.authorGöçmen, Bayram
dc.contributor.authorSüssmuth, Roderich D.
dc.contributor.authorCalderón-Celis, Francisco
dc.contributor.authorNosti, Alicia Jiménez
dc.contributor.authorEncinar, Jorge Ruiz
dc.contributor.otherAmerican
dc.date.accessioned2023-09-15T11:07:16Z
dc.date.available2023-09-15T11:07:16Z
dc.date.issued2021-10-04
dc.description.abstractWe report a novel hybrid, molecular and elemental mass spectrometry (MS) setup for the absolute quantification of snake venom proteomes shown here for two desert black cobra species within the genus Walterinnesia, Walterinnesia aegyptia and Walterinnesia morgani. The experimental design includes the decomplexation of the venom samples by reverse-phase chromatography independently coupled to four mass spectrometry systems: the combined bottom-up and top-down molecular MS for protein identification and a parallel reverse-phase microbore high-performance liquid chromatograph (RP-μHPLC) on-line to inductively coupled plasma (ICP-MS/MS) elemental mass spectrometry and electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QToF MS). This allows to continuously record the absolute sulfur concentration throughout the chromatogram and assign it to the parent venom proteins separated in the RP-μHPLC-ESI-QToF parallel run via mass profiling. The results provide a locus-resolved and quantitative insight into the three desert black cobra venom proteome samples. They also validate the units of measure of our snake venomics strategy for the relative quantification of snake venom proteomes as % of total venom peptide bonds as a proxy for the % by weight of the venom toxins/toxin families. In a more general context, our work may pave the way for broader applications of hybrid elemental/molecular MS setups in diverse areas of proteomics.en
dc.identifier.eissn1535-3907
dc.identifier.issn1535-3893
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/20027
dc.identifier.urihttps://doi.org/10.14279/depositonce-18825
dc.language.isoen
dc.publisherAmerican Chemical Society (ACS)
dc.relation.ispartof10.14279/depositonce-18526
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc500 Naturwissenschaften und Mathematik::540 Chemie::543 Analytische Chemie
dc.subject.ddc500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::572 Biochemie
dc.subject.othersnake venomicsen
dc.subject.othercombined top-down and bottom-up venomicsen
dc.subject.otherhybrid elemental and molecular mass spectrometryen
dc.subject.otherabsolute quantification of venom proteomeen
dc.subject.otherdesert black cobraen
dc.subject.otherWalterinnesia aegyptiaen
dc.subject.otherWalterinnesia morganien
dc.titleCombined Molecular and Elemental Mass Spectrometry Approaches for Absolute Quantification of Proteomes: Application to the Venomics Characterization of the Two Species of Desert Black Cobras, Walterinnesia aegyptia and Walterinnesia morganien
dc.typeArticle
dc.type.versionpublishedVersion
dcterms.bibliographicCitation.doi10.1021/acs.jproteome.1c00608
dcterms.bibliographicCitation.issue11
dcterms.bibliographicCitation.journaltitleJournal of Proteome Research
dcterms.bibliographicCitation.originalpublishernameAmerican Chemical Society
dcterms.bibliographicCitation.originalpublisherplaceWashington, DC
dcterms.bibliographicCitation.pageend5078
dcterms.bibliographicCitation.pagestart5064
dcterms.bibliographicCitation.volume20
dcterms.rightsHolder.referenceCreative-Commons-Lizenz
tub.accessrights.dnbfree
tub.affiliationFak. 2 Mathematik und Naturwissenschaften::Inst. Chemie::FG Biologische Chemie
tub.publisher.universityorinstitutionTechnische Universität Berlin

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