Comparison of mosquito and fly derived DNA as a tool for sampling vertebrate biodiversity in suburban forests in Berlin, Germany

dc.contributor.authorDanabalan, Renita
dc.contributor.authorPlanillo, Aimara
dc.contributor.authorButschkau, Susanne
dc.contributor.authorDeeg, Sita
dc.contributor.authorPierre, Gras
dc.contributor.authorThion, Cincia
dc.contributor.authorCalvignac‐Spencer, Sébastien
dc.contributor.authorKramer‐Schadt, Stephanie
dc.contributor.authorMazzoni, Camila
dc.date.accessioned2023-08-10T15:55:39Z
dc.date.available2023-08-10T15:55:39Z
dc.date.issued2023-02-22
dc.date.updated2023-07-19T07:56:57Z
dc.description.abstractThe use of invertebrate-derived DNA (iDNA) is a promising non-invasive tool to monitor wildlife. While most studies have been carried out in dense tropical and sub-tropical forests and have focused on the use of a single category of invertebrates, this study compares the use of flies and mosquitoes-derived DNA to assess vertebrate diversity in semi-urban environments. We conducted our sampling in four different forest plots in Berlin, Germany. Pools of flies and non-bloodfed mosquitoes were metabarcoded using 108-bp vertebrate-specific 12 S rRNA (12 S-V5) and 94-bp mammal-specific 16 S rRNA (16Smam) mitochondrial markers, and individual bloodfed mosquitoes were sequenced using the 340-bp vertebrate-specific 12 S rRNA fragment (Mam-12 S-340). Most sequencing was only successful for mammal species. From the fly pools, we detected 10 mammal species using 16Smam, and six species using 12 S-V5. From the non-bloodfed mosquito pools, we only amplified putative contaminant DNA, indicating that mosquito females without visual signs of a blood meal carry no traces of vertebrate DNA. Finally, in the bloodfed mosquitoes, we identified four mammal species. We did not find significant differences in the proportion of mammal species detected regarding the total available number of species between sampling localities. Fly samples were easier to obtain and more abundant over the sampled localities compared to mosquito samples. We conclude that, while there are a few advantages in using mosquito blood meals, the use of flies in the detection of wildlife in a suburban environment is more effective in terms of collection of samples and detection of vertebrates, although this technique is limited to few mammal species in the urban environment.en
dc.description.sponsorshipBMBF, 01LC1501, Bridging in Biodiversity Science - BIBS
dc.identifier.eissn2637-4943
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/19545
dc.identifier.urihttps://doi.org/10.14279/depositonce-18344
dc.language.isoen
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subject.ddc500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::577 Ökologie
dc.subject.otherDNA metabarcoding
dc.subject.otheriDNA
dc.subject.othermammals
dc.subject.othermosquito bloodmeal
dc.subject.othernon‐invasive sampling
dc.subject.otherurban ecology
dc.titleComparison of mosquito and fly derived DNA as a tool for sampling vertebrate biodiversity in suburban forests in Berlin, Germanyen
dc.typeArticle
dc.type.versionpublishedVersion
dcterms.bibliographicCitation.doi10.1002/edn3.398
dcterms.bibliographicCitation.issue3
dcterms.bibliographicCitation.journaltitleEnvironmental DNAen
dcterms.bibliographicCitation.originalpublishernameWiley
dcterms.bibliographicCitation.originalpublisherplaceNew York, NY
dcterms.bibliographicCitation.pageend487
dcterms.bibliographicCitation.pagestart476
dcterms.bibliographicCitation.volume5
dcterms.rightsHolder.referenceCreative-Commons-Lizenz
tub.accessrights.dnbfree
tub.affiliationFak. 6 Planen Bauen Umwelt::Inst. Ökologie::FG Planungsbezogene Tierökologie
tub.publisher.universityorinstitutionTechnische Universität Berlin

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