Genome-wide expression analysis upon constitutive activation of the HacA bZIP transcription factor in Aspergillus niger reveals a coordinated cellular response to counteract ER stress

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dc.contributor.authorCarvalho, Neuzaen
dc.contributor.authorJorgensen, Thomasen
dc.contributor.authorArentshorst, Marken
dc.contributor.authorNitsche, Benjaminen
dc.contributor.authorHondel, Cees A. M. J. J. van denen
dc.contributor.authorArcher, Daviden
dc.contributor.authorRam, Arthuren
dc.date.accessioned2015-11-21T00:41:43Z
dc.date.available2015-06-25T12:00:00Z
dc.date.issued2012
dc.date.submitted2015-06-25
dc.description.abstractBackground: HacA/Xbp1 is a conserved bZIP transcription factor in eukaryotic cells which regulates gene expression in response to various forms of secretion stress and as part of secretory cell differentiation. In the present study, we replaced the endogenous hacA gene of an Aspergillus niger strain with a gene encoding a constitutively active form of the HacA transcription factor (HacACA). The impact of constitutive HacA activity during exponential growth was explored in bioreactor controlled cultures using transcriptomic analysis to identify affected genes and processes. Results: Transcription profiles for the wild-type strain (HacAWT) and the HacACA strain were obtained using Affymetrix GeneChip analysis of three replicate batch cultures of each strain. In addition to the well known HacA targets such as the ER resident foldases and chaperones, GO enrichment analysis revealed up-regulation of genes involved in protein glycosylation, phospholipid biosynthesis, intracellular protein transport, exocytosis and protein complex assembly in the HacACA mutant. Biological processes over-represented in the down-regulated genes include those belonging to central metabolic pathways, translation and transcription. A remarkable transcriptional response in the HacACA strain was the down-regulation of the AmyR transcription factor and its target genes. Conclusions: The results indicate that the constitutive activation of the HacA leads to a coordinated regulation of the folding and secretion capacity of the cell, but with consequences on growth and fungal physiology to reduce secretion stress.en
dc.identifier.eissn1471-2164
dc.identifier.uriurn:nbn:de:kobv:83-opus4-67698
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/4805
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-4508
dc.languageEnglishen
dc.language.isoenen
dc.rights.urihttps://creativecommons.org/licenses/by/2.0/en
dc.subject.ddc570 Biowissenschaften; Biologieen
dc.subject.ddc610 Medizin und Gesundheiten
dc.subject.otherAspergillus nigeren
dc.subject.otherHacAen
dc.subject.otherprotein secretionen
dc.subject.otherRESSen
dc.subject.othersecretion stressen
dc.subject.otherunfolded protein responseen
dc.subject.otherXBP1en
dc.titleGenome-wide expression analysis upon constitutive activation of the HacA bZIP transcription factor in Aspergillus niger reveals a coordinated cellular response to counteract ER stressen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.articlenumber350en
dcterms.bibliographicCitation.doi10.1186/1471-2164-13-350en
dcterms.bibliographicCitation.journaltitleBMC Genomicsen
dcterms.bibliographicCitation.originalpublishernameBioMed Centralen
dcterms.bibliographicCitation.originalpublisherplaceLondonen
dcterms.bibliographicCitation.volume13en
tub.accessrights.dnbfree*
tub.affiliationFak. 3 Prozesswissenschaften::Inst. Biotechnologiede
tub.affiliation.facultyFak. 3 Prozesswissenschaftende
tub.affiliation.instituteInst. Biotechnologiede
tub.identifier.opus46769
tub.publisher.universityorinstitutionTechnische Universität Berlinen

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