Fabrication, in vitro and in vivo studies of bilayer composite membrane for periodontal guided tissue regeneration

dc.contributor.authorZahid, Saba
dc.contributor.authorKhan, Abdul Samad
dc.contributor.authorChaudhry, Aqif Anwar
dc.contributor.authorGhafoor, Sarah
dc.contributor.authorAin, Qurat Ul
dc.contributor.authorRaza, Ahtasham
dc.contributor.authorRahim, Muhammad Imran
dc.contributor.authorGörke, Oliver
dc.contributor.authorRehman, Ihtesham Ur
dc.contributor.authorTufail, Asma
dc.date.accessioned2020-11-12T09:41:50Z
dc.date.available2020-11-12T09:41:50Z
dc.date.issued2018-12-03
dc.date.updated2020-05-13T13:18:20Z
dc.descriptionThis publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.en
dc.descriptionDieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.de
dc.description.abstractDevelopment of a guided occlusive biodegradable membrane with controlled morphology in order to restrict the ingrowth of epithelial cells is still a challenge in dental tissue engineering. A bilayer membrane with a non-porous upper layer (polyurethane) and porous lower layer (polycaprolactone and bioactive glass composite) with thermoelastic properties to sustain surgery treatment was developed by lyophilization. Morphology, porosity, and layers attachment were controlled by using the multi-solvent system. In vitro and in vivo biocompatibility, cell attachment, and cell proliferation were analyzed by immunohistochemistry and histology. The cell proliferation rate and cell attachment results showed good biocompatibility of both surfaces, though cell metabolic activity was better on the polycaprolactone-bioactive glass surface. Furthermore, the cells were viable, adhered, and proliferated well on the lower porous bioactive surface, while non-porous polyurethane surface demonstrated low cell attachment, which was deliberately designed and a pre-requisite for guided tissue regeneration/guided bone regeneration membranes. In addition, in vivo studies performed in a rat model for six weeks revealed good compatibility of membranes. Histological analysis (staining with hematoxylin and eosin) indicated no signs of inflammation or accumulation of host immune cells. These results suggested that the fabricated biocompatible bilayer membrane has the potential for use in periodontal tissue regeneration.en
dc.identifier.eissn1530-8022
dc.identifier.issn0885-3282
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/11908
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-10799
dc.language.isoenen
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.ddc610 Medizin und Gesundheitde
dc.subject.otherguided tissue regenerationen
dc.subject.otherbioactive glassen
dc.subject.otherbilayer membraneen
dc.subject.otherin vitro cytotoxicityen
dc.subject.otheranimal testingen
dc.subject.otherimmuno-stainingen
dc.titleFabrication, in vitro and in vivo studies of bilayer composite membrane for periodontal guided tissue regenerationen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.doi10.1177/0885328218814986en
dcterms.bibliographicCitation.issue7en
dcterms.bibliographicCitation.journaltitleJournal of Biomaterials Applicationsen
dcterms.bibliographicCitation.originalpublishernameSAGEen
dcterms.bibliographicCitation.originalpublisherplaceLondonen
dcterms.bibliographicCitation.pageend978en
dcterms.bibliographicCitation.pagestart967en
dcterms.bibliographicCitation.volume33en
tub.accessrights.dnbdomain*
tub.affiliationFak. 3 Prozesswissenschaften>Inst. Werkstoffwissenschaften und -technologien>FG Keramische Werkstoffede
tub.affiliation.facultyFak. 3 Prozesswissenschaftende
tub.affiliation.groupFG Keramische Werkstoffede
tub.affiliation.instituteInst. Werkstoffwissenschaften und -technologiende
tub.publisher.universityorinstitutionTechnische Universität Berlinen
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