Physiological analysis oflactobacillus rhamnosusVTT E‐97800

dc.contributor.authorSunny‐Roberts, Elizabeth Oluwaseun
dc.contributor.authorKnorr, Dietrich
dc.date.accessioned2019-12-11T10:44:50Z
dc.date.available2019-12-11T10:44:50Z
dc.date.issued2007-09-11
dc.descriptionDieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.de
dc.descriptionThis publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.en
dc.description.abstractPurpose – This paper aims to describe the physiological analysis of L. rhamnosus VTT E‐97800 and its adaptive response to osmotic stress induced by trehalose. Design/methodology/approach – Cells of L. rhamnosus E800 in the stationary phase of growth were subjected to osmotic stress induced by trehalose treatments. The effects of osmotic stress on the viability of the study strain were determined by conducting flow cytometric analysis with carboxyfluorescein diacetate (cFDA) and propidium iodide (PI) and by observing the corresponding cells growth on MRS agar plates. Osmotic‐induced changes of esterase activity and membrane integrity were monitored. Ability to extrude intracellular accumulated cF (additional vitality marker) was taken into consideration. Findings – The fluorescence‐based approach gave additional insights on osmotic induced changes of cellular events, which could not be explicitly assessed by culture techniques. Trehalose treatments caused a transient membrane permeabilization as revealed by a gradual decrease in esterase activity (a measure of enzyme activity and thus of viability) with increase in trehalose molarity. However, culturability on MRS agar was not significantly affected. Membrane integrity was maintained and there was an improvement in the ability of cells to extrude intracellular accumulated cF. Originality/value – The paper provides a comparative study of the conventional culture techniques and the flow cytometric viability assessment which showed that esterase activity cannot be relied on to ascertain the culturability and viability status of an organism.en
dc.identifier.eissn1758-4108
dc.identifier.issn0007-070X
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/10471
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-9423
dc.language.isoen
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subject.ddc630 Landwirtschaft und verwandte Bereichede
dc.subject.ddc640 Hauswirtschaft und Familiede
dc.subject.ddc660 Chemische Verfahrenstechnikde
dc.subject.otherfood preservationen
dc.subject.othermetabolic diseasesen
dc.subject.othermicro-organismsen
dc.subject.otherbiotechnologyen
dc.titlePhysiological analysis oflactobacillus rhamnosusVTT E‐97800en
dc.title.subtitleAdaptive response to osmotic stress induced by trehaloseen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.doi10.1108/00070700710780706
dcterms.bibliographicCitation.issue9
dcterms.bibliographicCitation.journaltitleBritish Food Journalen
dcterms.bibliographicCitation.originalpublishernameEmeralden
dcterms.bibliographicCitation.originalpublisherplaceBingley en
dcterms.bibliographicCitation.pageend748
dcterms.bibliographicCitation.pagestart735
dcterms.bibliographicCitation.volume109
tub.accessrights.dnbdomain
tub.affiliationFak. 3 Prozesswissenschaften::Inst. Lebensmitteltechnologie und Lebensmittelchemie::FG Lebensmittelbiotechnologie und -prozesstechnikde
tub.affiliation.facultyFak. 3 Prozesswissenschaftende
tub.affiliation.groupFG Lebensmittelbiotechnologie und -prozesstechnikde
tub.affiliation.instituteInst. Lebensmitteltechnologie und Lebensmittelchemiede
tub.publisher.universityorinstitutionTechnische Universität Berlinde

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