Dendronylation: Residue-specific chemoselective attachment of oligoglycerol dendrimers on proteins with noncanonical amino acids
| dc.contributor.author | Ma, Ying | |
| dc.contributor.author | Thota, Bala N. S. | |
| dc.contributor.author | Haag, Rainer | |
| dc.contributor.author | Budisa, Nediljko | |
| dc.date.accessioned | 2018-05-07T08:32:52Z | |
| dc.date.available | 2018-05-07T08:32:52Z | |
| dc.date.issued | 2015 | |
| dc.description.abstract | Polyglycerol dendrimers as an important class of polymeric materials especially attractive for covalent attachment to therapeutic proteins as a useful alternative to traditional PEGylation procedures. Herein, we combine in vivo noncanonical amino acid (ncAA) incorporation and chemoselective conjugation in vitro to produce novel hybrid protein–dendrimer conjugates with the defined architectures. We incorporated Azidohomoalanine (Aha) as methionine substitute in vivo into various protein scaffolds to allow non-invasive dendrimer conjugations (dendronylation). Our approach makes recombinant proteins accessible for the design of multivalent dendrimer conjugates since it enables the preparation of many sequences with various positions for regioselective dendronylation. | en |
| dc.identifier.issn | 0960-894X | |
| dc.identifier.uri | https://depositonce.tu-berlin.de/handle/11303/7752 | |
| dc.identifier.uri | http://dx.doi.org/10.14279/depositonce-6930 | |
| dc.language.iso | en | en |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject.ddc | 540 Chemie und zugeordnete Wissenschaften | de |
| dc.subject.other | click-chemistry | en |
| dc.subject.other | noncanonical amino acids | en |
| dc.subject.other | oligoglycerol dendrons | en |
| dc.subject.other | protein–dendron conjugate | en |
| dc.subject.other | unnatural protein expression | en |
| dc.title | Dendronylation: Residue-specific chemoselective attachment of oligoglycerol dendrimers on proteins with noncanonical amino acids | en |
| dc.type | Article | en |
| dc.type.version | publishedVersion | en |
| dcterms.bibliographicCitation.doi | 10.1016/j.bmcl.2015.09.055 | en |
| dcterms.bibliographicCitation.issue | 22 | en |
| dcterms.bibliographicCitation.journaltitle | Bioorganic and Medicinal Chemistry Letters | en |
| dcterms.bibliographicCitation.originalpublishername | Elsevier | en |
| dcterms.bibliographicCitation.originalpublisherplace | Amsterdam | en |
| dcterms.bibliographicCitation.pageend | 5249 | en |
| dcterms.bibliographicCitation.pagestart | 5247 | en |
| dcterms.bibliographicCitation.volume | 25 | en |
| tub.accessrights.dnb | free | en |
| tub.affiliation | Fak. 2 Mathematik und Naturwissenschaften::Inst. Chemie::FG Biokatalyse | de |
| tub.affiliation.faculty | Fak. 2 Mathematik und Naturwissenschaften | de |
| tub.affiliation.group | FG Biokatalyse | de |
| tub.affiliation.institute | Inst. Chemie | de |
| tub.publisher.universityorinstitution | Technische Universität Berlin | en |