Evolution of fluorinated enzymes: an emerging trend for biocatalyst stabilization

dc.contributor.authorBiava, Hernan
dc.contributor.authorBudisa, Nediljko
dc.date.accessioned2017-07-14T10:50:13Z
dc.date.available2017-07-14T10:50:13Z
dc.date.issued2014
dc.description.abstractNature uses remarkably limited sets of chemistries in its repertoire, especially when compared to synthetic organic chemistry. This limits both the chemical and structural diversity that can ultimately be achieved with biocatalysis, unless the powers of chemical synthesis are merged with biological systems by integrating nonnatural synthetic chemistries into the protoplasma of living cells. Of particular interest, here is the fluorous effect that has recently established the potential to generate enzymes with an increased resistance toward both high temperature and organic solvents. For these reasons, we are witnessing a rapid development of efficient methodologies for the incorporation of fluorinated amino acids in protein synthesis, using both in vivo and in vitro strategies. In this review, we highlight relevant and trendsetting results in the design and engineering of stable fluorinated proteins and peptides along with whole-cell biocatalysis as an economically attractive and convenient application with exclusive focus on industrial biocatalysis. Finally, we envision new strategies to improve current achievements and enable the field to progress far beyond the current state-of-the-art.en
dc.description.sponsorshipDFG, EXC 314, Unifying Concepts in Catalysisen
dc.identifier.eissn1618-2863
dc.identifier.issn1618-0240
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/6499
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-6007
dc.language.isoen
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc660 Chemische Verfahrenstechnikde
dc.subject.otherbiocatalysisen
dc.subject.otherenzyme stabilizationen
dc.subject.otherfluorinated amino acidsen
dc.subject.otherfluorinationen
dc.subject.otherfluorous effecten
dc.titleEvolution of fluorinated enzymes: an emerging trend for biocatalyst stabilizationen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.doi10.1002/elsc.201300049
dcterms.bibliographicCitation.issue4
dcterms.bibliographicCitation.journaltitleEngineering in life sciencesen
dcterms.bibliographicCitation.originalpublishernameWiley-VCHen
dcterms.bibliographicCitation.originalpublisherplaceWeinheimen
dcterms.bibliographicCitation.pageend351
dcterms.bibliographicCitation.pagestart340
dcterms.bibliographicCitation.volume14
tub.accessrights.dnbfree
tub.affiliationFak. 2 Mathematik und Naturwissenschaften::Inst. Chemie::FG Biokatalysede
tub.affiliation.facultyFak. 2 Mathematik und Naturwissenschaftende
tub.affiliation.groupFG Biokatalysede
tub.affiliation.instituteInst. Chemiede
tub.publisher.universityorinstitutionTechnische Universität Berlin

Files

Original bundle
Now showing 1 - 1 of 1
Loading…
Thumbnail Image
Name:
10.1002.elsc.201300049.pdf
Size:
959.76 KB
Format:
Adobe Portable Document Format

Collections