Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella
| dc.contributor.author | Savas, Sumeyra | |
| dc.contributor.author | Ersoy, Aylin | |
| dc.contributor.author | Gulmez, Yakup | |
| dc.contributor.author | Kilic, Selcuk | |
| dc.contributor.author | Levent, Belkis | |
| dc.contributor.author | Altintas, Zeynep | |
| dc.date.accessioned | 2019-09-11T15:57:23Z | |
| dc.date.available | 2019-09-11T15:57:23Z | |
| dc.date.issued | 2018-08-27 | |
| dc.date.updated | 2019-08-01T02:35:54Z | |
| dc.description.abstract | Bacteria-related pathogenic diseases are one of the major health problems throughout the world. Salmonella is a genus of rod-shaped Gram-negative enterobacteria of which more than 2600 serotypes have been identified. Infection with Salmonella can cause salmonellosis, a serious bacterial toxi-infection syndrome associated with gastroenteritis, and paralyphoid and typhoid fevers. Its rapid and sensitive detection is a key to the prevention of problems related to health. This paper describes the development of antibody and DNA sensors for Salmonella detection using a microfluidic-based electrochemical system. Commercial Salmonella typhimurium and Salmonella typhimurium from human stool samples were investigated using standard and nanomaterial-amplified antibody sensors. S. typhimurium could be detected down to 1 cfu mL−1. The specificity of immunoassay was tested by studying with non-specific bacteria including E. coli and S. aureus that revealed only 2.01% and 2.66% binding when compared to the target bacterium. On the other hand, the quantification of Salmonella DNA was investigated in a concentration range of 0.002–200 µM using the developed DNA biosensor that demonstrated very high specificity and sensitivity with a detection limit of 0.94 nM. Our custom-designed microfluidic sensor offers rapid, highly sensitive, and specific diagnostic assay approaches for pathogen detection. | en |
| dc.identifier.eissn | 1996-1944 | |
| dc.identifier.uri | https://depositonce.tu-berlin.de/handle/11303/10001 | |
| dc.identifier.uri | http://dx.doi.org/10.14279/depositonce-8992 | |
| dc.language.iso | en | en |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject.ddc | 540 Chemie und zugeordnete Wissenschaften | de |
| dc.subject.other | Salmonella spp. | en |
| dc.subject.other | pathogen detection | en |
| dc.subject.other | antibody biosensor | en |
| dc.subject.other | DNA biosensor | en |
| dc.subject.other | microfluidic-based electrochemical sensor | en |
| dc.subject.other | nanoparticle enhanced bio-detection | en |
| dc.subject.other | infectious diseases | en |
| dc.title | Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella | en |
| dc.type | Article | en |
| dc.type.version | publishedVersion | en |
| dcterms.bibliographicCitation.articlenumber | 1541 | en |
| dcterms.bibliographicCitation.doi | 10.3390/ma11091541 | en |
| dcterms.bibliographicCitation.issue | 9 | en |
| dcterms.bibliographicCitation.journaltitle | Materials | en |
| dcterms.bibliographicCitation.originalpublishername | MDPI | en |
| dcterms.bibliographicCitation.originalpublisherplace | Basel | en |
| dcterms.bibliographicCitation.volume | 11 | en |
| tub.accessrights.dnb | free | en |
| tub.affiliation | Fak. 2 Mathematik und Naturwissenschaften::Inst. Chemie::FG Technische Chemie | de |
| tub.affiliation.faculty | Fak. 2 Mathematik und Naturwissenschaften | de |
| tub.affiliation.group | FG Technische Chemie | de |
| tub.affiliation.institute | Inst. Chemie | de |
| tub.publisher.universityorinstitution | Technische Universität Berlin | en |