Kinetic Analysis of the Hydrolysis of Pentose‐1‐phosphates through Apparent Nucleoside Phosphorolysis Equilibrium Shifts

dc.contributor.authorKaspar, Felix
dc.contributor.authorNeubauer, Peter
dc.contributor.authorKurreck, Anke
dc.date.accessioned2021-02-17T13:27:34Z
dc.date.available2021-02-17T13:27:34Z
dc.date.issued2020-12-04
dc.date.updated2021-02-15T14:55:21Z
dc.description.abstractHerein, we report an addition to the toolbox for the monitoring and quantification of the hydrolytic decay of pentose‐1‐phosphates, which are known to be elusive and difficult to quantify. This communication describes how apparent equilibrium shifts of a nucleoside phosphorolysis reaction can be employed to calculate hydrolytic loss of pentose‐1‐phosphates based on the measurement of post‐hydrolysis equilibrium concentrations of a nucleoside and a nucleobase. To demonstrate this approach, we assessed the stability of the relatively stable ribose‐1‐phosphate at 98 °C and found half‐lives of 1.8–11.7 h depending on the medium pH. This approach can be extended to other sugar phosphates and related reaction systems to quantify the stability of UV‐inactive and hard‐to‐detect reaction products and intermediates.en
dc.description.sponsorshipTU Berlin, Open-Access-Mittel – 2020en
dc.identifier.eissn1439-7641
dc.identifier.issn1439-4235
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/12625
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-11434
dc.language.isoenen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subject.ddc540 Chemie und zugeordnete Wissenschaftende
dc.subject.otherdecayen
dc.subject.otherphosphateen
dc.subject.otherriboseen
dc.subject.othersugar phosphateen
dc.subject.otherUV spectroscopyen
dc.titleKinetic Analysis of the Hydrolysis of Pentose‐1‐phosphates through Apparent Nucleoside Phosphorolysis Equilibrium Shiftsen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.doi10.1002/cphc.202000901en
dcterms.bibliographicCitation.issue3en
dcterms.bibliographicCitation.journaltitleChemPhysChemen
dcterms.bibliographicCitation.originalpublishernameWileyen
dcterms.bibliographicCitation.originalpublisherplaceNew York, NYen
dcterms.bibliographicCitation.pageend287en
dcterms.bibliographicCitation.pagestart283en
dcterms.bibliographicCitation.volume22en
tub.accessrights.dnbfreeen
tub.affiliationFak. 3 Prozesswissenschaften>Inst. Biotechnologie>FG Bioverfahrenstechnikde
tub.affiliation.facultyFak. 3 Prozesswissenschaftende
tub.affiliation.groupFG Bioverfahrenstechnikde
tub.affiliation.instituteInst. Biotechnologiede
tub.publisher.universityorinstitutionTechnische Universität Berlinen
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