Direct electron irradiation of DNA in a fully aqueous environment. Damage determination in combination with Monte Carlo simulations

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dc.contributor.authorHahn, Marc Benjamin
dc.contributor.authorMeyer, Susann
dc.contributor.authorSchröter, Maria-Astrid
dc.contributor.authorSeitz, Harald
dc.contributor.authorKunte, Hans-Jörg
dc.contributor.authorSolomun, Tihomir
dc.contributor.authorSturm, Heinz
dc.date.accessioned2018-04-19T08:47:32Z
dc.date.available2018-04-19T08:47:32Z
dc.date.issued2016
dc.description.abstractWe report on a study in which plasmid DNA in water was irradiated with 30 keV electrons generated by a scanning electron microscope and passed through a 100 nm thick Si3N4 membrane. The corresponding Monte Carlo simulations suggest that the kinetic energy spectrum of the electrons throughout the water is dominated by low energy electrons (<100 eV). The DNA radiation damage, single-strand breaks (SSBs) and double-strand breaks (DSBs), was determined by gel electrophoresis. The median lethal dose of D1/2 = 1.7 ± 0.3 Gy was found to be much smaller as compared to partially or fully hydrated DNA irradiated under vacuum conditions. The ratio of the DSBs to SSBs was found to be 1 : 12 as compared to 1 : 88 found for hydrated DNA. Our method enables quantitative measurements of radiation damage to biomolecules (DNA, proteins) in solutions under varying conditions (pH, salinity, co-solutes) for an electron energy range which is difficult to probe by standard methods.en
dc.identifier.eissn1463-9084
dc.identifier.issn1463-9076
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/7642
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-6832
dc.language.isoen
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.subject.ddc540 Chemie und zugeordnete Wissenschaften
dc.titleDirect electron irradiation of DNA in a fully aqueous environment. Damage determination in combination with Monte Carlo simulationsen
dc.typeArticle
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.doi10.1039/c6cp07707b
dcterms.bibliographicCitation.issue3
dcterms.bibliographicCitation.journaltitlePhysical Chemistry Chemical Physicsen
dcterms.bibliographicCitation.originalpublishernameRoyal Society of Chemistry (RSC)
dcterms.bibliographicCitation.originalpublisherplaceCambridge
dcterms.bibliographicCitation.pageend1805
dcterms.bibliographicCitation.pagestart1798
dcterms.bibliographicCitation.volume19
tub.accessrights.dnbfree
tub.affiliationFak. 5 Verkehrs- und Maschinensysteme::Inst. Werkzeugmaschinen und Fabrikbetrieb::FG Tribologiede
tub.affiliation.facultyFak. 5 Verkehrs- und Maschinensystemede
tub.affiliation.groupFG Tribologiede
tub.affiliation.instituteInst. Werkzeugmaschinen und Fabrikbetriebde
tub.publisher.universityorinstitutionTechnische Universität Berlinde

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