Conditional expression of the small GTPase ArfA impacts secretion, morphology, growth, and actin ring position in Aspergillus niger

dc.contributor.authorFiedler, Markus R. M.
dc.contributor.authorCairns, Timothy C.
dc.contributor.authorKoch, Oliver
dc.contributor.authorKubisch, Christin
dc.contributor.authorMeyer, Vera
dc.date.accessioned2018-05-17T08:00:20Z
dc.date.available2018-05-17T08:00:20Z
dc.date.issued2018-05-08
dc.description.abstractIn filamentous fungi, growth and protein secretion occurs predominantly at the tip of long, thread like cells termed hyphae. This requires coordinated regulation of multiple processes, including vesicle trafficking, exocytosis, and endocytosis, which are facilitated by a complex cytoskeletal apparatus. In this study, functional analyses of the small GTPase ArfA from Aspergillus niger demonstrate that this protein functionally complements the Saccharomyces cerevisiae ARF1/2, and that this protein is essential for A. niger. Loss-of-function and gain-of-function analyses demonstrate that titration of arfA expression impacts hyphal growth rate, hyphal tip morphology, and protein secretion. Moreover, localization of the endocytic machinery, visualized via fluorescent tagging of the actin ring, was found to be abnormal in ArfA under- and overexpressed conditions. Finally, we provide evidence that the major secreted protein GlaA localizes at septal junctions, indicating that secretion in A. niger may occur at these loci, and that this process is likely impacted by arfA expression levels. Taken together, our results demonstrate that ArfA fulfills multiple functions in the secretory pathway of A. niger.en
dc.description.sponsorshipDFG, 325093850, Open Access Publizieren 2017 - 2018 / Technische Universität Berlinde
dc.identifier.issn1664-302X
dc.identifier.urihttps://depositonce.tu-berlin.de//handle/11303/7799
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-6977
dc.language.isoenen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subject.ddc570 Biowissenschaften; Biologiede
dc.subject.otherAspergillus nigeren
dc.subject.othertet-onen
dc.subject.otherarfen
dc.subject.otherprotein secretionen
dc.subject.otherglucoamylaseen
dc.subject.otherGTPaseen
dc.subject.otheractinen
dc.subject.otherconditional gene expressionen
dc.titleConditional expression of the small GTPase ArfA impacts secretion, morphology, growth, and actin ring position in Aspergillus nigeren
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.articlenumber878en
dcterms.bibliographicCitation.doi10.3389/fmicb.2018.00878en
dcterms.bibliographicCitation.journaltitleFrontiers in Microbiologyen
dcterms.bibliographicCitation.originalpublishernameFrontiers Mediaen
dcterms.bibliographicCitation.originalpublisherplaceLausanneen
dcterms.bibliographicCitation.pageend17en
dcterms.bibliographicCitation.pagestart1en
dcterms.bibliographicCitation.volume9en
tub.accessrights.dnbfreeen
tub.affiliationFak. 3 Prozesswissenschaften>Inst. Biotechnologie>FG Angewandte und Molekulare Mikrobiologiede
tub.affiliation.facultyFak. 3 Prozesswissenschaftende
tub.affiliation.groupFG Angewandte und Molekulare Mikrobiologiede
tub.affiliation.instituteInst. Biotechnologiede
tub.publisher.universityorinstitutionTechnische Universität Berlinen
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