Powering Artificial Enzymatic Cascades with Electrical Energy
| dc.contributor.author | Al‐Shameri, Ammar | |
| dc.contributor.author | Petrich, Marie‐Christine | |
| dc.contributor.author | junge Puring, Kai | |
| dc.contributor.author | Apfel, Ulf‐Peter | |
| dc.contributor.author | Nestl, Bettina M. | |
| dc.contributor.author | Lauterbach, Lars | |
| dc.date.accessioned | 2020-11-16T14:22:36Z | |
| dc.date.available | 2020-11-16T14:22:36Z | |
| dc.date.issued | 2020-04-28 | |
| dc.date.updated | 2020-11-02T11:25:20Z | |
| dc.description.abstract | We have developed a scalable platform that employs electrolysis for an in vitro synthetic enzymatic cascade in a continuous flow reactor. Both H2 and O2 were produced by electrolysis and transferred through a gas‐permeable membrane into the flow system. The membrane enabled the separation of the electrolyte from the biocatalysts in the flow system, where H2 and O2 served as electron mediators for the biocatalysts. We demonstrate the production of methylated N‐heterocycles from diamines with up to 99 % product formation as well as excellent regioselective labeling with stable isotopes. Our platform can be applied for a broad panel of oxidoreductases to exploit electrical energy for the synthesis of fine chemicals. | en |
| dc.description.sponsorship | DFG, 284111627, H2-basierende Kaskaden für die Biosynthese von N-Heterocyclen | en |
| dc.description.sponsorship | TU Berlin, Open-Access-Mittel – 2020 | en |
| dc.description.sponsorship | DFG, 390540038, EXC 2008: Unifying Systems in Catalysis "UniSysCat" | en |
| dc.description.sponsorship | DFG, 390677874, EXC 2033: RESOLV (Ruhr Explores Solvation) | en |
| dc.identifier.eissn | 1521-3757 | |
| dc.identifier.issn | 0044-8249 | |
| dc.identifier.uri | https://depositonce.tu-berlin.de/handle/11303/11973 | |
| dc.identifier.uri | http://dx.doi.org/10.14279/depositonce-10855 | |
| dc.language.iso | en | en |
| dc.relation.ispartof | 10.14279/depositonce-10645 | en |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject.ddc | 540 Chemie und zugeordnete Wissenschaften | de |
| dc.subject.other | electrochemical biocatalysis | en |
| dc.subject.other | hydrogenases | en |
| dc.subject.other | imine reductases | en |
| dc.subject.other | isotopic labeling | en |
| dc.subject.other | N-heterocycles | en |
| dc.title | Powering Artificial Enzymatic Cascades with Electrical Energy | en |
| dc.type | Article | en |
| dc.type.version | publishedVersion | en |
| dcterms.bibliographicCitation.doi | 10.1002/anie.202001302 | en |
| dcterms.bibliographicCitation.issue | 27 | en |
| dcterms.bibliographicCitation.journaltitle | Angewandte Chemie | en |
| dcterms.bibliographicCitation.originalpublishername | Wiley | en |
| dcterms.bibliographicCitation.originalpublisherplace | New York, NY | en |
| dcterms.bibliographicCitation.pageend | 10933 | en |
| dcterms.bibliographicCitation.pagestart | 10929 | en |
| dcterms.bibliographicCitation.volume | 59 | en |
| tub.accessrights.dnb | free | en |
| tub.affiliation | Fak. 2 Mathematik und Naturwissenschaften::Inst. Chemie::FG Physikalische Chemie / Biophysikalische Chemie | de |
| tub.affiliation.faculty | Fak. 2 Mathematik und Naturwissenschaften | de |
| tub.affiliation.group | FG Physikalische Chemie / Biophysikalische Chemie | de |
| tub.affiliation.institute | Inst. Chemie | de |
| tub.publisher.universityorinstitution | Technische Universität Berlin | en |