Generation of a 3D Liver Model Comprising Human Extracellular Matrix in an Alginate/Gelatin-Based Bioink by Extrusion Bioprinting for Infection and Transduction Studies

dc.contributor.authorHiller, Thomas
dc.contributor.authorBerg, Johanna
dc.contributor.authorElomaa, Laura
dc.contributor.authorRöhrs, Viola
dc.contributor.authorUllah, Imran
dc.contributor.authorSchaar, Katrin
dc.contributor.authorDietrich, Ann-Christin
dc.contributor.authorAl-Zeer, Munir A.
dc.contributor.authorKurtz, Andreas
dc.contributor.authorHocke, Andreas C.
dc.contributor.authorHippenstiel, Stefan
dc.contributor.authorFechner, Henry
dc.contributor.authorWeinhart, Marie
dc.contributor.authorKurreck, Jens
dc.date.accessioned2018-11-05T11:10:04Z
dc.date.available2018-11-05T11:10:04Z
dc.date.issued2018-10-12
dc.description.abstractBioprinting is a novel technology that may help to overcome limitations associated with two-dimensional (2D) cell cultures and animal experiments, as it allows the production of three-dimensional (3D) tissue models composed of human cells. The present study describes the optimization of a bioink composed of alginate, gelatin and human extracellular matrix (hECM) to print human HepaRG liver cells with a pneumatic extrusion printer. The resulting tissue model was tested for its suitability for the study of transduction by an adeno-associated virus (AAV) vector and infection with human adenovirus 5 (hAdV5). We found supplementation of the basic alginate/gelatin bioink with 0.5 and 1 mg/mL hECM provides desirable properties for the printing process, the stability of the printed constructs, and the viability and metabolic functions of the printed HepaRG cells. The tissue models were efficiently transduced by AAV vectors of serotype 6, which successfully silenced an endogenous target (cyclophilin B) by means of RNA interference. Furthermore, the printed 3D model supported efficient adenoviral replication making it suitable to study virus biology and develop new antiviral compounds. We consider the approach described here paradigmatic for the development of 3D tissue models for studies including viral vectors and infectious viruses.en
dc.description.sponsorshipDFG, 325093850, Open Access Publizieren 2017 - 2018 / Technische Universität Berlinde
dc.identifier.eissn1422-0067
dc.identifier.urihttps://depositonce.tu-berlin.de//handle/11303/8409
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-7558
dc.language.isoenen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subject.ddc570 Biowissenschaften; Biologiede
dc.subject.otheradeno-associated virusen
dc.subject.otheradenovirusen
dc.subject.otherbioprintingen
dc.subject.otherinfectionen
dc.subject.othertransductionen
dc.subject.otherextracellular matrixen
dc.subject.otherliveren
dc.subject.otherorgan modelsen
dc.subject.otherHepaRGen
dc.subject.othergene silencingen
dc.titleGeneration of a 3D Liver Model Comprising Human Extracellular Matrix in an Alginate/Gelatin-Based Bioink by Extrusion Bioprinting for Infection and Transduction Studiesen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.articlenumber3129en
dcterms.bibliographicCitation.doi10.3390/ijms19103129en
dcterms.bibliographicCitation.issue10en
dcterms.bibliographicCitation.journaltitleInternational Journal of Molecular Sciencesen
dcterms.bibliographicCitation.originalpublishernameMDPIen
dcterms.bibliographicCitation.originalpublisherplaceBaselen
dcterms.bibliographicCitation.volume19en
tub.accessrights.dnbfreeen
tub.affiliationFak. 3 Prozesswissenschaften>Inst. Biotechnologie>FG Angewandte Biochemiede
tub.affiliation.facultyFak. 3 Prozesswissenschaftende
tub.affiliation.groupFG Angewandte Biochemiede
tub.affiliation.instituteInst. Biotechnologiede
tub.publisher.universityorinstitutionTechnische Universität Berlinen
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