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Mapping local electric fields in proteins at biomimetic interfaces

Schkolnik, Gal; Utesch, Tillmann; Salewski, Johannes; Tenger, Katalin; Millo, Diego; Kranich, Anja; Zebger, Ingo; Schulz, Claudia; Zimányi, László; Rákhely, Gábór; Mroginski, Maria Andrea; Hildebrandt, Peter

We present a novel approach for determining the strength of the electric field experienced by proteins immobilised on membrane models. It is based on the vibrational Stark effect of a nitrile label introduced at different positions on engineered proteins and monitored by surface enhanced infrared absorption spectroscopy.
Published in: Chemical communications, 10.1039/c1cc13186a, Royal Society of Chemistry
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