A dynamic multi-organ-chip for long-term cultivation and substance testing proven by 3D human liver and skin tissue co-culture

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dc.contributor.authorWagner, Ilka
dc.contributor.authorMaterne, Eva-Maria
dc.contributor.authorBrincker, Sven
dc.contributor.authorSüßbier, Ute
dc.contributor.authorFrädrich, Caroline
dc.contributor.authorBusek, Mathias
dc.contributor.authorSonntag, Frank
dc.contributor.authorSakharov, Dmitry A.
dc.contributor.authorTrushkin, Evgeny V.
dc.contributor.authorTonevitsky, Alexander G.
dc.contributor.authorLauster, Roland
dc.contributor.authorMarx, Uwe
dc.date.accessioned2016-06-24T05:35:14Z
dc.date.available2016-06-24T05:35:14Z
dc.date.issued2013
dc.descriptionDieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.de
dc.descriptionThis publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.en
dc.description.abstractCurrent in vitro and animal tests for drug development are failing to emulate the systemic organ complexity of the human body and, therefore, to accurately predict drug toxicity. In this study, we present a multi-organ-chip capable of maintaining 3D tissues derived from cell lines, primary cells and biopsies of various human organs. We designed a multi-organ-chip with co-cultures of human artificial liver microtissues and skin biopsies, each a 1/100 000 of the biomass of their original human organ counterparts, and have successfully proven its long-term performance. The system supports two different culture modes: i) tissue exposed to the fluid flow, or ii) tissue shielded from the underlying fluid flow by standard Transwell® cultures. Crosstalk between the two tissues was observed in 14-day co-cultures exposed to fluid flow. Applying the same culture mode, liver microtissues showed sensitivity at different molecular levels to the toxic substance troglitazone during a 6-day exposure. Finally, an astonishingly stable long-term performance of the Transwell®-based co-cultures could be observed over a 28-day period. This mode facilitates exposure of skin at the air–liquid interface. Thus, we provide here a potential new tool for systemic substance testing.en
dc.description.sponsorshipBMBF, 0315569, GO-Bio 3: Multi-Organ-Bioreaktoren für die prädiktive Substanztestung im Chipformaten
dc.identifier.eissn1473-0197
dc.identifier.pmid23648632
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/5643
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-5263
dc.language.isoen
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.ddc004 Datenverarbeitung; Informatikde
dc.subject.ddc570 Biowissenschaften; Biologiede
dc.subject.ddc540 Chemie und zugeordnete Wissenschaftende
dc.titleA dynamic multi-organ-chip for long-term cultivation and substance testing proven by 3D human liver and skin tissue co-cultureen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.doi10.1039/c3lc50234a
dcterms.bibliographicCitation.issue18
dcterms.bibliographicCitation.journaltitleLab on a chip : miniaturisation for chemistry and biologyen
dcterms.bibliographicCitation.originalpublishernameRoyal Society of Chemistryde
dcterms.bibliographicCitation.originalpublisherplaceCambridgede
dcterms.bibliographicCitation.pageend3547
dcterms.bibliographicCitation.pagestart3538
dcterms.bibliographicCitation.volume13
tub.accessrights.dnbdomain
tub.affiliationFak. 3 Prozesswissenschaften::Inst. Biotechnologie::FG Medizinische Biotechnologiede
tub.affiliation.facultyFak. 3 Prozesswissenschaftende
tub.affiliation.groupFG Medizinische Biotechnologiede
tub.affiliation.instituteInst. Biotechnologiede
tub.publisher.universityorinstitutionTechnische Universität Berlin

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