Automated Laser‐Transfer Synthesis of High‐Density Microarrays for Infectious Disease Screening

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dc.contributor.authorParis, Grigori
dc.contributor.authorHeidepriem, Jasmin
dc.contributor.authorTsouka, Alexandra
dc.contributor.authorLiu, Yuxin
dc.contributor.authorMattes, Daniela S.
dc.contributor.authorPinzón Martín, Sandra
dc.contributor.authorDallabernardina, Pietro
dc.contributor.authorMende, Marco
dc.contributor.authorLindner, Celina
dc.contributor.authorWawrzinek, Robert
dc.contributor.authorRademacher, Christoph
dc.contributor.authorSeeberger, Peter H.
dc.contributor.authorBreitling, Frank
dc.contributor.authorBischoff, Frank Ralf
dc.contributor.authorWolf, Timo
dc.contributor.authorLoeffler, Felix F.
dc.date.accessioned2023-01-18T13:25:50Z
dc.date.available2023-01-18T13:25:50Z
dc.date.issued2022-04-15
dc.date.updated2022-09-21T21:45:09Z
dc.description.abstractLaser‐induced forward transfer (LIFT) is a rapid laser‐patterning technique for high‐throughput combinatorial synthesis directly on glass slides. A lack of automation and precision limits LIFT applications to simple proof‐of‐concept syntheses of fewer than 100 compounds. Here, an automated synthesis instrument is reported that combines laser transfer and robotics for parallel synthesis in a microarray format with up to 10 000 individual reactions cm−2. An optimized pipeline for amide bond formation is the basis for preparing complex peptide microarrays with thousands of different sequences in high yield with high reproducibility. The resulting peptide arrays are of higher quality than commercial peptide arrays. More than 4800 15‐residue peptides resembling the entire Ebola virus proteome on a microarray are synthesized to study the antibody response of an Ebola virus infection survivor. Known and unknown epitopes that serve now as a basis for Ebola diagnostic development are identified. The versatility and precision of the synthesizer is demonstrated by in situ synthesis of fluorescent molecules via Schiff base reaction and multi‐step patterning of precisely definable amounts of fluorophores. This automated laser transfer synthesis approach opens new avenues for high‐throughput chemical synthesis and biological screening.en
dc.description.sponsorshipBMBF, 13XP5050A, Erforschung einer neuen Methode für die Herstellung von hochdichten Molekülbibliotheken (cLIFT)
dc.identifier.eissn1521-4095
dc.identifier.issn0935-9648
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/18007
dc.identifier.urihttps://doi.org/10.14279/depositonce-16799
dc.language.isoen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc540 Chemie und zugeordnete Wissenschaftende
dc.subject.otherhigh‐throughput
dc.subject.otherlaser‐induced forward transfer
dc.subject.otherpeptides
dc.subject.otherSchiff base fluorophores
dc.subject.othersolid phase synthesis
dc.titleAutomated Laser‐Transfer Synthesis of High‐Density Microarrays for Infectious Disease Screeningen
dc.typeArticle
dc.type.versionpublishedVersion
dcterms.bibliographicCitation.articlenumber2200359
dcterms.bibliographicCitation.doi10.1002/adma.202200359
dcterms.bibliographicCitation.issue23
dcterms.bibliographicCitation.journaltitleAdvanced Materialsen
dcterms.bibliographicCitation.originalpublishernameWiley
dcterms.bibliographicCitation.originalpublisherplaceNew York, NY
dcterms.bibliographicCitation.volume34
dcterms.rightsHolder.referenceCreative-Commons-Lizenz
tub.accessrights.dnbfree
tub.affiliationFak. 5 Verkehrs- und Maschinensysteme::Inst. Mechanik::FG Systemdynamik und Reibungsphysik
tub.publisher.universityorinstitutionTechnische Universität Berlin

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