Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms
| dc.contributor.author | Peebo, Karl | |
| dc.contributor.author | Neubauer, Peter | |
| dc.date.accessioned | 2019-08-23T14:34:11Z | |
| dc.date.available | 2019-08-23T14:34:11Z | |
| dc.date.issued | 2018-06-21 | |
| dc.date.updated | 2019-08-01T00:00:32Z | |
| dc.description.abstract | Depending on the environmental conditions, cells adapt their metabolism and specific growth rate. Rearrangements occur on many different levels such as macromolecular composition, gene and protein expression, morphology and metabolic flux patterns. As the interplay of these processes also determines the output of a recombinant protein producing system, having control over specific growth rate of the culture is advantageous. Continuous culture methods were developed to grow cells in a constant environment and have been used for decades to study basic microbial physiology in a controlled and reproducible manner. Our review summarizes the uses of continuous cultures in cell physiology studies and process development, with a focus on recombinant protein-producing microorganisms. | en |
| dc.identifier.eissn | 2076-2607 | |
| dc.identifier.uri | https://depositonce.tu-berlin.de/handle/11303/9880 | |
| dc.identifier.uri | http://dx.doi.org/10.14279/depositonce-8892 | |
| dc.language.iso | en | en |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject.ddc | 570 Biowissenschaften; Biologie | de |
| dc.subject.other | continuous cultures | en |
| dc.subject.other | continuous manufacturing | en |
| dc.subject.other | recombinant protein production | en |
| dc.subject.other | steady state | en |
| dc.subject.other | specific growth rate | en |
| dc.subject.other | chemostat | en |
| dc.title | Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms | en |
| dc.type | Article | en |
| dc.type.version | publishedVersion | en |
| dcterms.bibliographicCitation.articlenumber | 56 | en |
| dcterms.bibliographicCitation.doi | 10.3390/microorganisms6030056 | en |
| dcterms.bibliographicCitation.issue | 3 | en |
| dcterms.bibliographicCitation.journaltitle | Microorganisms | en |
| dcterms.bibliographicCitation.originalpublishername | MDPI | en |
| dcterms.bibliographicCitation.originalpublisherplace | Basel | en |
| dcterms.bibliographicCitation.volume | 6 | en |
| tub.accessrights.dnb | free | en |
| tub.affiliation | Fak. 3 Prozesswissenschaften::Inst. Biotechnologie::FG Bioverfahrenstechnik | de |
| tub.affiliation.faculty | Fak. 3 Prozesswissenschaften | de |
| tub.affiliation.group | FG Bioverfahrenstechnik | de |
| tub.affiliation.institute | Inst. Biotechnologie | de |
| tub.publisher.universityorinstitution | Technische Universität Berlin | en |