Functionalized peptide hydrogels as tunable extracellular matrix mimics for biological applications

dc.contributor.authorHellmund, Katharina S.
dc.contributor.authorvon Lospichl, Benjamin
dc.contributor.authorBöttcher, Christoph
dc.contributor.authorLudwig, Kai
dc.contributor.authorKeiderling, Uwe
dc.contributor.authorNoirez, Laurence
dc.contributor.authorWeiß, Annika
dc.contributor.authorMikolajczak, Dorian J.
dc.contributor.authorGradzielski, Michael
dc.contributor.authorKoksch, Beate
dc.date.accessioned2021-04-06T12:04:18Z
dc.date.available2021-04-06T12:04:18Z
dc.date.issued2020-10-26
dc.date.updated2021-02-15T12:01:53Z
dc.description.abstractThe development of tailorable and biocompatible three‐dimensional (3D) substrates or molecular networks that reliably mimic the extracellular matrix (ECM) and influence cell behavior and growth in vitro is of increasing interest for cell‐based applications in the field of tissue engineering and regenerative medicine. In this context, we present a novel coiled coil‐based peptide that self‐assembles into a 3D‐α‐helical fibril network and functions as a self‐supporting hydrogel. By functionalizing distinct coiled‐coil peptides with cellular binding motifs or carbohydrate ligands (mannose), and by utilizing the multivalency and modularity of coiled‐coil assemblies, tailored artificial ECMs are obtained. Fibrillar network and ligand density, as well as ligand composition can readily be adjusted by changes in water content or peptide concentrations, respectively. Mesoscopic structure of these networks was assessed by rheology and small‐angle neutron scattering experiments. Initial cell viability studies using NIH/3T3 cells showed comparable or even superior cell viability using the presented artificial ECMs, compared to commercially available 3D‐cell culture scaffold Matrigel. The herein reported approach presents a reliable (low batch‐to‐batch variation) and modular pathway toward biocompatible and tailored artificial ECMs.en
dc.description.sponsorshipDFG, 32049920, Multivalenz in der Chemie und Biochemie (MGK)en
dc.identifier.eissn2475-8817
dc.identifier.issn1097-0282
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/12930
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-11731
dc.language.isoenen
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subject.ddc540 Chemie und zugeordnete Wissenschaftende
dc.subject.othercoiled coilen
dc.subject.otherextracellular matrixen
dc.subject.otherpeptide hydrogelen
dc.subject.otherSANSen
dc.titleFunctionalized peptide hydrogels as tunable extracellular matrix mimics for biological applicationsen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.articlenumbere24201en
dcterms.bibliographicCitation.doi10.1002/pep2.24201en
dcterms.bibliographicCitation.issue2en
dcterms.bibliographicCitation.journaltitlePeptide Scienceen
dcterms.bibliographicCitation.originalpublishernameWileyen
dcterms.bibliographicCitation.originalpublisherplaceNew York, NYen
dcterms.bibliographicCitation.volume113en
tub.accessrights.dnbfreeen
tub.affiliationFak. 2 Mathematik und Naturwissenschaften::Inst. Chemie::FG Physikalische Chemie / Molekulare Materialwissenschaftende
tub.affiliation.facultyFak. 2 Mathematik und Naturwissenschaftende
tub.affiliation.groupFG Physikalische Chemie / Molekulare Materialwissenschaftende
tub.affiliation.instituteInst. Chemiede
tub.publisher.universityorinstitutionTechnische Universität Berlinen

Files

Original bundle
Now showing 1 - 1 of 1
Loading…
Thumbnail Image
Name:
PEP2_PEP224201.pdf
Size:
3.49 MB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
4.9 KB
Format:
Item-specific license agreed upon to submission
Description:

Collections