Reliable identification of protein-protein interactions by crosslinking mass spectrometry

dc.contributor.authorLenz, Swantje
dc.contributor.authorSinn, Ludwig
dc.contributor.authorO'Reilly, Francis
dc.contributor.authorFischer, Lutz
dc.contributor.authorWegner, Fritz
dc.contributor.authorRappsilber, Juri
dc.date.accessioned2021-09-13T08:50:28Z
dc.date.available2021-09-13T08:50:28Z
dc.date.issued2021-06-11
dc.description.abstractProtein-protein interactions govern most cellular pathways and processes, and multiple technologies have emerged to systematically map them. Assessing the error of interaction networks has been a challenge. Crosslinking mass spectrometry is currently widening its scope from structural analyses of purified multi-protein complexes towards systems-wide analyses of protein-protein interactions (PPIs). Using a carefully controlled large-scale analysis of Escherichia coli cell lysate, we demonstrate that false-discovery rates (FDR) for PPIs identified by crosslinking mass spectrometry can be reliably estimated. We present an interaction network comprising 590 PPIs at 1% decoy-based PPI-FDR. The structural information included in this network localises the binding site of the hitherto uncharacterised protein YacL to near the DNA exit tunnel on the RNA polymerase.en
dc.description.sponsorshipTU Berlin, Open-Access-Mittel – 2021en
dc.description.sponsorshipDFG, 390540038, EXC 2008: Unifying Systems in Catalysis "UniSysCat"en
dc.description.sponsorshipDFG, 392923329, GRK 2473: Bioaktive Peptide - Innovative Aspekte zur Synthese und Biosyntheseen
dc.description.sponsorshipDFG, 426290502, Erfassung der strukturellen Organisation des Mycoplasma pneumoniae Proteoms mittels in-Zell Crosslinking-Massenspektrometrieen
dc.identifier.eissn2041-1723
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/13523
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-12306
dc.language.isoenen
dc.relation.ispartof10.14279/depositonce-15535
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subject.ddc570 Biowissenschaften; Biologiede
dc.subject.otherprotein-protein interactionsen
dc.subject.othercrosslinking mass spectrometryen
dc.subject.otherstatistical methodsen
dc.subject.otherproteome informaticsen
dc.subject.otherstructural biologyen
dc.titleReliable identification of protein-protein interactions by crosslinking mass spectrometryen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.articlenumber3564en
dcterms.bibliographicCitation.doi10.1038/s41467-021-23666-zen
dcterms.bibliographicCitation.journaltitleNature communicationsen
dcterms.bibliographicCitation.originalpublishernameSpringer Natureen
dcterms.bibliographicCitation.originalpublisherplaceHeidelbergen
dcterms.bibliographicCitation.volume12en
tub.accessrights.dnbfreeen
tub.affiliationFak. 3 Prozesswissenschaften>Inst. Biotechnologie>FG Bioanalytikde
tub.affiliation.facultyFak. 3 Prozesswissenschaftende
tub.affiliation.groupFG Bioanalytikde
tub.affiliation.instituteInst. Biotechnologiede
tub.publisher.universityorinstitutionTechnische Universität Berlinen
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