Reliable identification of protein-protein interactions by crosslinking mass spectrometry
| dc.contributor.author | Lenz, Swantje | |
| dc.contributor.author | Sinn, Ludwig | |
| dc.contributor.author | O'Reilly, Francis | |
| dc.contributor.author | Fischer, Lutz | |
| dc.contributor.author | Wegner, Fritz | |
| dc.contributor.author | Rappsilber, Juri | |
| dc.date.accessioned | 2021-09-13T08:50:28Z | |
| dc.date.available | 2021-09-13T08:50:28Z | |
| dc.date.issued | 2021-06-11 | |
| dc.description.abstract | Protein-protein interactions govern most cellular pathways and processes, and multiple technologies have emerged to systematically map them. Assessing the error of interaction networks has been a challenge. Crosslinking mass spectrometry is currently widening its scope from structural analyses of purified multi-protein complexes towards systems-wide analyses of protein-protein interactions (PPIs). Using a carefully controlled large-scale analysis of Escherichia coli cell lysate, we demonstrate that false-discovery rates (FDR) for PPIs identified by crosslinking mass spectrometry can be reliably estimated. We present an interaction network comprising 590 PPIs at 1% decoy-based PPI-FDR. The structural information included in this network localises the binding site of the hitherto uncharacterised protein YacL to near the DNA exit tunnel on the RNA polymerase. | en |
| dc.description.sponsorship | TU Berlin, Open-Access-Mittel – 2021 | en |
| dc.description.sponsorship | DFG, 390540038, EXC 2008: Unifying Systems in Catalysis "UniSysCat" | en |
| dc.description.sponsorship | DFG, 392923329, GRK 2473: Bioaktive Peptide - Innovative Aspekte zur Synthese und Biosynthese | en |
| dc.description.sponsorship | DFG, 426290502, Erfassung der strukturellen Organisation des Mycoplasma pneumoniae Proteoms mittels in-Zell Crosslinking-Massenspektrometrie | en |
| dc.identifier.eissn | 2041-1723 | |
| dc.identifier.uri | https://depositonce.tu-berlin.de/handle/11303/13523 | |
| dc.identifier.uri | http://dx.doi.org/10.14279/depositonce-12306 | |
| dc.language.iso | en | en |
| dc.relation.ispartof | 10.14279/depositonce-15535 | |
| dc.relation.ispartof | 10.14279/depositonce-16305 | |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject.ddc | 570 Biowissenschaften; Biologie | de |
| dc.subject.other | protein-protein interactions | en |
| dc.subject.other | crosslinking mass spectrometry | en |
| dc.subject.other | statistical methods | en |
| dc.subject.other | proteome informatics | en |
| dc.subject.other | structural biology | en |
| dc.title | Reliable identification of protein-protein interactions by crosslinking mass spectrometry | en |
| dc.type | Article | en |
| dc.type.version | publishedVersion | en |
| dcterms.bibliographicCitation.articlenumber | 3564 | en |
| dcterms.bibliographicCitation.doi | 10.1038/s41467-021-23666-z | en |
| dcterms.bibliographicCitation.journaltitle | Nature communications | en |
| dcterms.bibliographicCitation.originalpublishername | Springer Nature | en |
| dcterms.bibliographicCitation.originalpublisherplace | Heidelberg | en |
| dcterms.bibliographicCitation.volume | 12 | en |
| tub.accessrights.dnb | free | en |
| tub.affiliation | Fak. 3 Prozesswissenschaften::Inst. Biotechnologie::FG Bioanalytik | de |
| tub.affiliation.faculty | Fak. 3 Prozesswissenschaften | de |
| tub.affiliation.group | FG Bioanalytik | de |
| tub.affiliation.institute | Inst. Biotechnologie | de |
| tub.publisher.universityorinstitution | Technische Universität Berlin | en |