Competition of Pyridoxal 5'-Phosphate with Ribulose 1,5-Bisphosphate and Effector Sugar Phosphates at the Reaction Centers of the Spinach Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase

dc.contributor.authorVater, Joachim
dc.contributor.authorGaudszun, Thomas
dc.contributor.authorScharnow, Harald
dc.contributor.authorSalnikow, Johann
dc.date.accessioned2019-01-04T13:17:37Z
dc.date.available2019-01-04T13:17:37Z
dc.date.issued1980
dc.description.abstractThe Stimulation of the carboxylase reaction by effectors of ribulose 1,5-bisphosphate carboxyl­ ase/oxygenase displays higher sensitivity towards pyridoxal 5'-phosphate inhibition than the catalytical process itself. Pyridoxal 5'-phosphate binding to the enzyme is not affected by the modulators 6-phospho-gluconate and fructose 1,6-bisphosphate at low concentrations at which these agents stimulate the carboxylation rate. At higher concentrations these sugar phosphates protect the enzyme against pyridoxal 5'-phos-phate inhibition in a similar fashion like the substrate ribulose 1,5-bisphosphate. Such protection experiments in combination with spectrophotometrical studies of pyridoxal 5'-phosphate binding demonstrate two binding states of ribulose 1,5-bisphosphate at the reaction centers of the enzyme with different requirements for Mg2+. 6-Phosphogluconate functions as protector only in the presence of Mg2+. Our results imply a competition between pyridoxal 5'-phosphate and substrate or effector sugar phosphates at the reaction centers of the spinach carboxylase. It is proposed that the pyridoxal 5'-phosphate inhibition of the stimulatory activity of these effectors originates from a modification of the regulatory sites of the enzyme caused by pyridoxal 5'-phosphate binding to the catalytical sites.en
dc.identifier.eissn1865-7125
dc.identifier.issn0939-5075
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/8805
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-7934
dc.language.isoen
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc570 Biowissenschaften; Biologiede
dc.subject.otherribulose 1,5-bisphosphate carboxylase/oxygenaseen
dc.subject.othereffector studiesen
dc.subject.otherpyridoxal 5'-phosphate inhibitionen
dc.titleCompetition of Pyridoxal 5'-Phosphate with Ribulose 1,5-Bisphosphate and Effector Sugar Phosphates at the Reaction Centers of the Spinach Ribulose 1,5-Bisphosphate Carboxylase/Oxygenaseen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.doi10.1515/znc-1980-5-611
dcterms.bibliographicCitation.issue5-6
dcterms.bibliographicCitation.journaltitleZeitschrift für Naturforschung Cde
dcterms.bibliographicCitation.originalpublishernameDe Gruyteren
dcterms.bibliographicCitation.originalpublisherplaceBerlin
dcterms.bibliographicCitation.pageend422
dcterms.bibliographicCitation.pagestart416
dcterms.bibliographicCitation.volume35
tub.accessrights.dnbfree
tub.affiliationFak. 2 Mathematik und Naturwissenschaften::Inst. Chemie::FG Physikalische Chemie / Biophysikalische Chemiede
tub.affiliation.facultyFak. 2 Mathematik und Naturwissenschaftende
tub.affiliation.groupFG Physikalische Chemie / Biophysikalische Chemiede
tub.affiliation.instituteInst. Chemiede
tub.publisher.universityorinstitutionTechnische Universität Berlinde
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