dc.contributor.author	Hertwig, Christian
dc.contributor.author	Steins, Veronika
dc.contributor.author	Reineke, Kai
dc.contributor.author	Rademacher, Antje
dc.contributor.author	Klocke, Michael
dc.contributor.author	Rauh, Cornelia
dc.contributor.author	Schlüter, Oliver
dc.date.accessioned	2019-10-30T11:13:36Z
dc.date.available	2019-10-30T11:13:36Z
dc.date.issued	2015-08-06
dc.date.updated	2019-09-29T06:58:26Z
dc.description.abstract	This study investigated the inactivation efficiency of cold atmospheric pressure plasma treatment on Bacillus subtilis endospores dependent on the used feed gas composition and on the surface, the endospores were attached on. Glass petri-dishes, glass beads, and peppercorns were inoculated with the same endospore density and treated with a radio frequency plasma jet. Generated reactive species were detected using optical emission spectroscopy. A quantitative polymerase chain reaction (qPCR) based ratio detection system was established to monitor the DNA damage during the plasma treatment. Argon + 0.135% vol. oxygen + 0.2% vol. nitrogen as feed gas emitted the highest amounts of UV-C photons and considerable amount of reactive oxygen and nitrogen species. Plasma generated with argon + 0.135% vol. oxygen was characterized by the highest emission of reactive oxygen species (ROS), whereas the UV-C emission was negligible. The use of pure argon showed a negligible emission of UV photons and atomic oxygen, however, the emission of vacuum (V)UV photons was assumed. Similar maximum inactivation results were achieved for the three feed gas compositions. The surface structure had a significant impact on the inactivation efficiency of the plasma treatment. The maximum inactivation achieved was between 2.4 and 2.8 log10 on glass petri-dishes and 3.9 to 4.6 log10 on glass beads. The treatment of peppercorns resulted in an inactivation lower than 1.0 log10. qPCR results showed a significant DNA damage for all gas compositions. Pure argon showed the highest results for the DNA damage ratio values, followed by argon + 0.135% vol. oxygen + 0.2% vol. nitrogen. In case of argon + 0.135% vol. oxygen the inactivation seems to be dominated by the action of ROS. These findings indicate the significant role of VUV and UV photons in the inactivation process of B. subtilis endospores.	en
dc.description.sponsorship	BEML, 2819102713, Plasma-basierte Dekontamination von trockenen pflanzlichen Produkten zur Erhöhung der Lebensmittelsicherheit (3Plas)	en
dc.identifier.eissn	1664-302X
dc.identifier.uri	https://depositonce.tu-berlin.de/handle/11303/10228
dc.identifier.uri	http://dx.doi.org/10.14279/depositonce-9189
dc.language.iso	en	en
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	en
dc.subject.ddc	660 Chemische Verfahrenstechnik	de
dc.subject.other	cold plasma	en
dc.subject.other	spore inactivation	en
dc.subject.other	inactivation mechanism	en
dc.subject.other	DNA damage	en
dc.subject.other	qPCR	en
dc.title	Impact of surface structure and feed gas composition on Bacillus subtilis endospore inactivation during direct plasma treatment	en
dc.type	Article	en
dc.type.version	publishedVersion	en
dcterms.bibliographicCitation.articlenumber	774	en
dcterms.bibliographicCitation.doi	10.3389/fmicb.2015.00774	en
dcterms.bibliographicCitation.journaltitle	Frontiers in Microbiology	en
dcterms.bibliographicCitation.originalpublishername	Frontiers Media S.A.	en
dcterms.bibliographicCitation.originalpublisherplace	Lausanne	en
dcterms.bibliographicCitation.volume	6	en
tub.accessrights.dnb	free	en
tub.affiliation	Fak. 3 Prozesswissenschaften::Inst. Lebensmitteltechnologie und Lebensmittelchemie::FG Lebensmittelbiotechnologie und -prozesstechnik	de
tub.affiliation.faculty	Fak. 3 Prozesswissenschaften	de
tub.affiliation.group	FG Lebensmittelbiotechnologie und -prozesstechnik	de
tub.affiliation.institute	Inst. Lebensmitteltechnologie und Lebensmittelchemie	de
tub.publisher.universityorinstitution	Technische Universität Berlin	en

Impact of surface structure and feed gas composition on Bacillus subtilis endospore inactivation during direct plasma treatment

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