Degradation Kinetics of Lignocellulolytic Enzymes in a Biogas Reactor Using Quantitative Mass Spectrometry

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dc.contributor.authorKüchler, Jan
dc.contributor.authorWillenbücher, Katharina
dc.contributor.authorReiß, Elisabeth
dc.contributor.authorNuß, Lea
dc.contributor.authorConrady, Marius
dc.contributor.authorRamm, Patrice
dc.contributor.authorSchimpf, Ulrike
dc.contributor.authorReichl, Udo
dc.contributor.authorSzewzyk, Ulrich
dc.contributor.authorBenndorf, Dirk
dc.date.accessioned2023-02-06T12:41:30Z
dc.date.available2023-02-06T12:41:30Z
dc.date.issued2023-01-12
dc.date.updated2023-02-03T19:14:17Z
dc.description.abstractThe supplementation of lignocellulose-degrading enzymes can be used to enhance the performance of biogas production in industrial biogas plants. Since the structural stability of these enzyme preparations is essential for efficient application, reliable methods for the assessment of enzyme stability are crucial. Here, a mass-spectrometric-based assay was established to monitor the structural stability of enzymes, i.e., the structural integrity of these proteins, in anaerobic digestion (AD). The analysis of extracts of Lentinula edodes revealed the rapid degradation of lignocellulose-degrading enzymes, with an approximate half-life of 1.5 h. The observed low structural stability of lignocellulose-degrading enzymes in AD corresponded with previous results obtained for biogas content. The established workflow can be easily adapted for the monitoring of other enzyme formulations and provides a platform for evaluating the effects of enzyme additions in AD, together with a characterization of the biochemical methane potential used in order to determine the biodegradability of organic substrates.
dc.identifier.eissn2311-5637
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/18150
dc.identifier.urihttps://doi.org/10.14279/depositonce-16943
dc.language.isoen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc570 Biowissenschaften; Biologiede
dc.subject.othermass spectrometry
dc.subject.otherbiogas production
dc.subject.otherfungal enzymes
dc.subject.otherlignocellulose conversion
dc.subject.otherLentinula edodes
dc.titleDegradation Kinetics of Lignocellulolytic Enzymes in a Biogas Reactor Using Quantitative Mass Spectrometry
dc.typeArticle
dc.type.versionpublishedVersion
dcterms.bibliographicCitation.articlenumber67
dcterms.bibliographicCitation.doi10.3390/fermentation9010067
dcterms.bibliographicCitation.issue1
dcterms.bibliographicCitation.journaltitleFermentation
dcterms.bibliographicCitation.originalpublishernameMDPI
dcterms.bibliographicCitation.originalpublisherplaceBasel
dcterms.bibliographicCitation.volume9
dcterms.rightsHolder.referenceCreative-Commons-Lizenz
tub.accessrights.dnbfree
tub.affiliationFak. 3 Prozesswissenschaften::Inst. Technischen Umweltschutz::FG Umweltmikrobiologie
tub.publisher.universityorinstitutionTechnische Universität Berlin

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