Quantitative phenotypic screens of Aspergillus niger mutants in solid and liquid culture

dc.contributor.authorCairns, Timothy
dc.contributor.authorZheng, Xiaomei
dc.contributor.authorFeurstein, Claudia
dc.contributor.authorZheng, Ping
dc.contributor.authorSun, Jibin
dc.contributor.authorMeyer, Vera
dc.date.accessioned2023-10-25T10:21:37Z
dc.date.available2023-10-25T10:21:37Z
dc.date.issued2022-11-24
dc.description.abstractThis protocol describes procedures for quantifying Aspergillus niger growth in both solid and liquid culture. Firstly, by comparing radial growth between mutant and progenitor isolates on solid agar supplemented with sublethal stressors, susceptibility coefficients can be calculated. Secondly, analysis of macromorphological growth types in liquid culture allows full quantification of how a gene of interest affects submerged growth. By combining these assays, an extensive and quantitative dataset of how a gene of interest impacts growth in this fungus is possible.en
dc.identifier.issn2666-1667
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/20382
dc.identifier.urihttps://doi.org/10.14279/depositonce-19180
dc.language.isoen
dc.publisherElsevier BV
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::579 Mikroorganismen, Pilze, Algen
dc.subject.othergeneticsen
dc.subject.othermicrobiologyen
dc.subject.othermodel organismsen
dc.subject.othermolecular biologyen
dc.subject.otherbiotechnologyen
dc.subject.otherbioengineeringen
dc.titleQuantitative phenotypic screens of Aspergillus niger mutants in solid and liquid culture
dc.typeArticle
dc.type.versionpublishedVersion
dcterms.bibliographicCitation.articlenumber101883
dcterms.bibliographicCitation.doi10.1016/j.xpro.2022.101883
dcterms.bibliographicCitation.issue4
dcterms.bibliographicCitation.journaltitleSTAR Protocols
dcterms.bibliographicCitation.originalpublishernameElsevier
dcterms.bibliographicCitation.originalpublisherplaceAmsterdam
dcterms.bibliographicCitation.volume3
dcterms.rightsHolder.referenceCreative-Commons-Lizenz
tub.accessrights.dnbfree
tub.affiliationFak. 3 Prozesswissenschaften::Inst. Biotechnologie::FG Angewandte und Molekulare Mikrobiologie
tub.publisher.universityorinstitutionTechnische Universität Berlin

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