Identification of the Unwinding Region in the Clostridioides difficile Chromosomal Origin of Replication

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dc.contributor.authorOliveira Paiva, Ana M.
dc.contributor.authorvan Eijk, Erika
dc.contributor.authorFriggen, Annemieke H.
dc.contributor.authorWeigel, Christoph
dc.contributor.authorSmits, Wiep Klaas
dc.date.accessioned2020-12-08T14:08:35Z
dc.date.available2020-12-08T14:08:35Z
dc.date.issued2020-10-02
dc.date.updated2020-10-02T09:28:40Z
dc.description.abstractFaithful DNA replication is crucial for viability of cells across all kingdoms. Targeting DNA replication is a viable strategy for inhibition of bacterial pathogens. Clostridioides difficile is an important enteropathogen that causes potentially fatal intestinal inflammation. Knowledge about DNA replication in this organism is limited and no data is available on the very first steps of DNA replication. Here, we use a combination of in silico predictions and in vitro experiments to demonstrate that C. difficile employs a bipartite origin of replication that shows DnaA-dependent melting at oriC2, located in the dnaA-dnaN intergenic region. Analysis of putative origins of replication in different clostridia suggests that the main features of the origin architecture are conserved. This study is the first to characterize aspects of the origin region of C. difficile and contributes to our understanding of the initiation of DNA replication in clostridia.en
dc.identifier.eissn1664-302X
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/12143
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-11017
dc.language.isoenen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subject.ddc570 Biowissenschaften; Biologiede
dc.subject.otheroriCen
dc.subject.otherClostridioides difficileen
dc.subject.otherP1 nucleaseen
dc.subject.otherunwindingen
dc.subject.otherDnaAen
dc.titleIdentification of the Unwinding Region in the Clostridioides difficile Chromosomal Origin of Replicationen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.articlenumber581401en
dcterms.bibliographicCitation.doi10.3389/fmicb.2020.581401en
dcterms.bibliographicCitation.journaltitleFrontiers in Microbiologyen
dcterms.bibliographicCitation.originalpublishernameFrontiersen
dcterms.bibliographicCitation.originalpublisherplaceLausanneen
dcterms.bibliographicCitation.volume11en
tub.accessrights.dnbfreeen
tub.affiliationFak. 3 Prozesswissenschaften::Inst. Biotechnologie::FG Bioverfahrenstechnikde
tub.affiliation.facultyFak. 3 Prozesswissenschaftende
tub.affiliation.groupFG Bioverfahrenstechnikde
tub.affiliation.instituteInst. Biotechnologiede
tub.publisher.universityorinstitutionTechnische Universität Berlinen

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