Application of a Rapid and Integrated Analysis System (RIAS) as a High-Throughput Processing Tool for In Vitro ADME Samples by Liquid Chromatography/Tandem Mass Spectrometry

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dc.contributor.authorLuippold, Andreas H.
dc.contributor.authorArnhold, Thomas
dc.contributor.authorJörg, Wolfgang
dc.contributor.authorKrüger, Beate
dc.contributor.authorSüßmuth, Roderich D.
dc.date.accessioned2019-01-08T17:41:27Z
dc.date.available2019-01-08T17:41:27Z
dc.date.issued2011
dc.descriptionDieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.de
dc.descriptionThis publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.en
dc.description.abstractOver the past decade, drug discovery programs have started to address the optimization of key ADME properties already at an early stage of the process. Hence, analytical chemists have been confronted with tremendously rising sample numbers and have had to develop methodologies accelerating quantitative liquid chromatography/tandem mass spectrometry (LC/MS/MS). This article focuses on the application of a generic and fully automated LC/MS/MS, named Rapid and Integrated Analysis System (RIAS), as a high-throughput platform for the rapid quantification of drug-like compounds in various in vitro ADME assays. Previous efforts were dedicated to the setup and feasibility study of a workflow-integrated platform combining a modified high-throughput liquid handling LC/MS/MS system controlled by a customized software interface and a customized data-processing and reporting tool. Herein the authors present an extension of this previously developed basic application to a broad set of ADME screening campaigns, covering CYP inhibition, Caco-2, and PAMPA assays. The platform is capable of switching automatically between various ADME assays, performs MS compound optimization if required, and provides a speed of 8 s from sample to sample, independently of the type of ADME assay. Quantification and peak review are adopted to the high-throughput environment and tested against a standard HPLC-ESI technology.en
dc.identifier.eissn1552-454X
dc.identifier.issn1087-0571
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/8904
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-8033
dc.language.isoen
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subject.ddc570 Biowissenschaften; Biologiede
dc.subject.otherhigh-throughputen
dc.subject.otherin vitro ADMEen
dc.subject.otheractive barcodingen
dc.subject.otherRIASen
dc.titleApplication of a Rapid and Integrated Analysis System (RIAS) as a High-Throughput Processing Tool for In Vitro ADME Samples by Liquid Chromatography/Tandem Mass Spectrometryen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.doi10.1177/1087057110397358
dcterms.bibliographicCitation.issue3
dcterms.bibliographicCitation.journaltitleJournal of Biomolecular Screeningen
dcterms.bibliographicCitation.originalpublishernameSAGE Publicationsen
dcterms.bibliographicCitation.originalpublisherplaceWashington, DCen
dcterms.bibliographicCitation.pageend377
dcterms.bibliographicCitation.pagestart370
dcterms.bibliographicCitation.volume16
tub.accessrights.dnbdomain
tub.affiliationFak. 2 Mathematik und Naturwissenschaften::Inst. Chemie::FG Biologische Chemiede
tub.affiliation.facultyFak. 2 Mathematik und Naturwissenschaftende
tub.affiliation.groupFG Biologische Chemiede
tub.affiliation.instituteInst. Chemiede
tub.publisher.universityorinstitutionTechnische Universität Berlinde

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