Proteomics of a fuzzy organelle: interphase chromatin
| dc.contributor.author | Kustatscher, Georg | |
| dc.contributor.author | Hégarat, Nadia | |
| dc.contributor.author | Wills, Karen L. H. | |
| dc.contributor.author | Furlan, Cristina | |
| dc.contributor.author | Bukowski-Wills, Jimi-Carlo | |
| dc.contributor.author | Hochegger, Helfrid | |
| dc.contributor.author | Rappsilber, Juri | |
| dc.date.accessioned | 2017-07-14T10:50:13Z | |
| dc.date.available | 2017-07-14T10:50:13Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | Chromatin proteins mediate replication, regulate expression, and ensure integrity of the genome. So far, a comprehensive inventory of interphase chromatin has not been determined. This is largely due to its heterogeneous and dynamic composition, which makes conclusive biochemical purification difficult, if not impossible. As a fuzzy organelle, it defies classical organellar proteomics and cannot be described by a single and ultimate list of protein components. Instead, we propose a new approach that provides a quantitative assessment of a protein's probability to function in chromatin. We integrate chromatin composition over a range of different biochemical and biological conditions. This resulted in interphase chromatin probabilities for 7635 human proteins, including 1840 previously uncharacterized proteins. We demonstrate the power of our large-scale data-driven annotation during the analysis of cyclin-dependent kinase (CDK) regulation in chromatin. Quantitative protein ontologies may provide a general alternative to list-based investigations of organelles and complement Gene Ontology. | en |
| dc.identifier.eissn | 1460-2075 | |
| dc.identifier.issn | 0261-4189 | |
| dc.identifier.pmid | 24534090 | |
| dc.identifier.uri | https://depositonce.tu-berlin.de/handle/11303/6500 | |
| dc.identifier.uri | http://dx.doi.org/10.14279/depositonce-6008 | |
| dc.language.iso | en | |
| dc.rights.uri | https://creativecommons.org/licenses/by/3.0/ | |
| dc.subject.ddc | 570 Biowissenschaften; Biologie | de |
| dc.subject.other | Cdk regulation | en |
| dc.subject.other | chromatin | en |
| dc.subject.other | machine learning | en |
| dc.subject.other | organelle | en |
| dc.subject.other | proteomics | en |
| dc.title | Proteomics of a fuzzy organelle: interphase chromatin | en |
| dc.type | Article | en |
| dc.type.version | publishedVersion | en |
| dcterms.bibliographicCitation.doi | 10.1002/embj.201387614 | |
| dcterms.bibliographicCitation.issue | 6 | |
| dcterms.bibliographicCitation.journaltitle | The EMBO journal | en |
| dcterms.bibliographicCitation.originalpublishername | Wiley | en |
| dcterms.bibliographicCitation.originalpublisherplace | Hoboken, NJ [u.a.] | en |
| dcterms.bibliographicCitation.pageend | 664 | |
| dcterms.bibliographicCitation.pagestart | 648 | |
| dcterms.bibliographicCitation.volume | 33 | |
| tub.accessrights.dnb | free | |
| tub.affiliation | Fak. 3 Prozesswissenschaften::Inst. Biotechnologie::FG Bioanalytik | de |
| tub.affiliation.faculty | Fak. 3 Prozesswissenschaften | de |
| tub.affiliation.group | FG Bioanalytik | de |
| tub.affiliation.institute | Inst. Biotechnologie | de |
| tub.publisher.universityorinstitution | Technische Universität Berlin |
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