Single-Use Printed Biosensor for L-Lactate and Its Application in Bioprocess Monitoring

dc.contributor.authorTheuer, Lorenz
dc.contributor.authorRandek, Judit
dc.contributor.authorJunne, Stefan
dc.contributor.authorNeubauer, Peter
dc.contributor.authorMandenius, Carl-Fredrik
dc.contributor.authorBeni, Valerio
dc.date.accessioned2020-05-12T06:36:54Z
dc.date.available2020-05-12T06:36:54Z
dc.date.issued2020-03-09
dc.date.updated2020-04-28T02:30:17Z
dc.description.abstractThere is a profound need in bioprocess manufacturing for low-cost single-use sensors that allow timely monitoring of critical product and production attributes. One such opportunity is screen-printed enzyme-based electrochemical sensors, which have the potential to enable low-cost online and/or off-line monitoring of specific parameters in bioprocesses. In this study, such a single-use electrochemical biosensor for lactate monitoring is designed and evaluated. Several aspects of its fabrication and use are addressed, including enzyme immobilization, stability, shelf-life and reproducibility. Applicability of the biosensor to off-line monitoring of bioprocesses was shown by testing in two common industrial bioprocesses in which lactate is a critical quality attribute (Corynebacterium fermentation and mammalian Chinese hamster ovary (CHO) cell cultivation). The specific response to lactate of the screen-printed biosensor was characterized by amperometric measurements. The usability of the sensor at typical industrial culture conditions was favorably evaluated and benchmarked with commonly used standard methods (HPLC and enzymatic kits). The single-use biosensor allowed fast and accurate detection of lactate in prediluted culture media used in industrial practice. The design and fabrication of the biosensor could most likely be adapted to several other critical bioprocess analytes using other specific enzymes. This makes this single-use screen-printed biosensor concept a potentially interesting and versatile tool for further applications in bioprocess monitoring.en
dc.description.sponsorshipEC/H2020/643056/EU/Rapid Bioprocess Development/Biorapiden
dc.identifier.eissn2227-9717
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/11117
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-10007
dc.language.isoenen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subject.ddc660 Chemische Verfahrenstechnikde
dc.subject.otherlactate biosensoren
dc.subject.otherenzyme electrodeen
dc.subject.otheroff-line monitoringen
dc.subject.otherscreen-printingen
dc.subject.otherat-line measurementen
dc.subject.otherin-line monitoringen
dc.titleSingle-Use Printed Biosensor for L-Lactate and Its Application in Bioprocess Monitoringen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.articlenumber321en
dcterms.bibliographicCitation.doi10.3390/pr8030321en
dcterms.bibliographicCitation.issue3en
dcterms.bibliographicCitation.journaltitleProcessesen
dcterms.bibliographicCitation.originalpublishernameMDPIen
dcterms.bibliographicCitation.originalpublisherplaceBaselen
dcterms.bibliographicCitation.volume8en
tub.accessrights.dnbfreeen
tub.affiliationFak. 3 Prozesswissenschaften>Inst. Biotechnologie>FG Bioverfahrenstechnikde
tub.affiliation.facultyFak. 3 Prozesswissenschaftende
tub.affiliation.groupFG Bioverfahrenstechnikde
tub.affiliation.instituteInst. Biotechnologiede
tub.publisher.universityorinstitutionTechnische Universität Berlinen
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