Crystallization of Intact and Subunit L-Deficient Monomers from Synechocystis PCC 6803 Photosystem I

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dc.contributor.authorJekow, Petra
dc.contributor.authorSchubert, Wolf-Dieter
dc.contributor.authorFromme, Petra
dc.contributor.authorKruip, Jochen
dc.contributor.authorChitnis, Parag R.
dc.contributor.authorRögner, Matthias
dc.contributor.authorSaenger, Wolfram
dc.date.accessioned2018-11-10T16:34:48Z
dc.date.available2018-11-10T16:34:48Z
dc.date.issued1996
dc.description.abstractPhotosystem I monomers from wildtype cells of Synechocystis PCC 6803 and from a mu­tant deficient in the psaL gene were crystallized. PsaL encodes for the hydrophobic subunit L, which has been proposed to constitute the trimerization domain in the PS I trimer. The absence of subunit L facilitated crystallization of the PS I monomer. The unit cell dimensions and the space group for the crystals from this preparation could be determined to be a = b = 132 Å , c -525 Å, α = β = 90°, y = 120°, the space group is P61 or P65. The results show the potential of using specifically designed deletion mutants of an integral membrane protein for the systematic improvement of crystal structure data.en
dc.identifier.eissn1865-7125
dc.identifier.issn0939-5075
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/8475
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-7621
dc.language.isoen
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc570 Biowissenschaften; Biologiede
dc.subject.otherPhotosystem Ien
dc.subject.otherPS I monomersen
dc.subject.othercrystallizationen
dc.subject.othermesophilic cyanobacteriumen
dc.titleCrystallization of Intact and Subunit L-Deficient Monomers from Synechocystis PCC 6803 Photosystem Ien
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.doi10.1515/znc-1996-3-410
dcterms.bibliographicCitation.issue3-4
dcterms.bibliographicCitation.journaltitleZeitschrift für Naturforschung Cde
dcterms.bibliographicCitation.originalpublishernameDe Gruyteren
dcterms.bibliographicCitation.originalpublisherplaceBerlinen
dcterms.bibliographicCitation.pageend199
dcterms.bibliographicCitation.pagestart195
dcterms.bibliographicCitation.volume51
tub.accessrights.dnbfree
tub.affiliationFak. 2 Mathematik und Naturwissenschaften::Inst. Chemiede
tub.affiliation.facultyFak. 2 Mathematik und Naturwissenschaftende
tub.affiliation.instituteInst. Chemiede
tub.publisher.universityorinstitutionTechnische Universität Berlinde

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