Emulating the early phases of human tooth development in vitro

dc.contributor.authorRosowski, Jennifer
dc.contributor.authorBräunig, Julia
dc.contributor.authorAmler, Anna-Klara
dc.contributor.authorStrietzel, Frank P.
dc.contributor.authorLauster, Roland
dc.contributor.authorRosowski, Mark
dc.date.accessioned2019-05-13T11:01:15Z
dc.date.available2019-05-13T11:01:15Z
dc.date.issued2019-05-07
dc.description.abstractFunctional in vitro models emulating the physiological processes of human organ formation are invaluable for future research and the development of regenerative therapies. Here, a developmentally inspired approach is pursued to reproduce fundamental steps of human tooth organogenesis in vitro using human dental pulp cells. Similar to the in vivo situation of tooth initiating mesenchymal condensation, a 3D self-organizing culture was pursued resulting in an organoid of the size of a human tooth germ with odontogenic marker expression. Furthermore, the model is capable of epithelial invagination into the condensed mesenchyme, mimicking the reciprocal tissue interactions of human tooth development. Comprehensive transcriptome analysis revealed activation of well-studied as well as rather less investigated signaling pathways implicated in human tooth organogenesis, such as the Notch signaling. Early condensation in vitro revealed a shift to the TGFß signal transduction pathway and a decreased RhoA small GTPase activity, connected to the remodeling of the cytoskeleton and actin-mediated mechanotransduction. Therefore, this in vitro model of tooth development provides a valuable model to study basic human developmental mechanisms.en
dc.description.sponsorshipDFG, 414044773, Open Access Publizieren 2019 - 2020 / Technische Universität Berlinen
dc.identifier.eissn2045-2322
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/9422
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-8480
dc.language.isoenen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subject.ddc500 Naturwissenschaften und Mathematikde
dc.subject.ddc600 Technik, Technologiede
dc.subject.otherstem-cell biotechnologyen
dc.subject.othertissue engineeringen
dc.titleEmulating the early phases of human tooth development in vitroen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.articlenumber7057en
dcterms.bibliographicCitation.doi10.1038/s41598-019-43468-0en
dcterms.bibliographicCitation.journaltitleScientific Reportsen
dcterms.bibliographicCitation.originalpublishernameSpringer Natureen
dcterms.bibliographicCitation.originalpublisherplaceBerlinen
dcterms.bibliographicCitation.volume9en
tub.accessrights.dnbfreeen
tub.affiliationFak. 3 Prozesswissenschaften::Inst. Biotechnologie::FG Medizinische Biotechnologiede
tub.affiliation.facultyFak. 3 Prozesswissenschaftende
tub.affiliation.groupFG Medizinische Biotechnologiede
tub.affiliation.instituteInst. Biotechnologiede
tub.publisher.universityorinstitutionTechnische Universität Berlinen

Files

Original bundle
Now showing 1 - 1 of 1
Loading…
Thumbnail Image
Name:
s41598-019-43468-0.pdf
Size:
6.4 MB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
4.9 KB
Format:
Item-specific license agreed upon to submission
Description:

Collections