Automated universal chip platform for fluorescence based cellular assays
| dc.contributor.author | Schmieder, F. | |
| dc.contributor.author | Schmieder, S. | |
| dc.contributor.author | Eger, R. | |
| dc.contributor.author | Friedrich, S. | |
| dc.contributor.author | Werner, A. | |
| dc.contributor.author | Danz, N. | |
| dc.contributor.author | Marx, Uwe | |
| dc.contributor.author | Sonntag, Frank | |
| dc.date.accessioned | 2017-11-28T13:26:15Z | |
| dc.date.available | 2017-11-28T13:26:15Z | |
| dc.date.issued | 2012 | |
| dc.description | Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich. | de |
| dc.description | This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively. | en |
| dc.description.abstract | The advantage of cell based assays used as biosensors is the direct access to hardly obtainable parameters like toxicity, mutagenicity and pharmacological effectiveness. Within the last few years we established a micro fluidic platform including a peristaltic micro pump as well as several valves, manifolds and micro channels [1]. For optical online monitoring the micro fluidic system is bonded to a glass slide. Furthermore the biochip is fixed on an electrically heated support. The pneumatically actuated peristaltic pump as well as the temperature control is performed by a control device. For the fluorescence based online monitoring a robotic guided fluorescence measurement module was developed, which supports the detection of fluorescence in microtiter plates and microfluidic systems. This measurement module allows the fluorescence detection of two different excitation / detection wavelengths (480 / 530 nm and 570 / 620 nm) and was successfully characterised using EGFP and Rhodamine 6G. Additionally three cell based assays with bacterial, yeast and human cells were characterized. | en |
| dc.identifier.eissn | 0013-5585 | |
| dc.identifier.issn | 1862-278X | |
| dc.identifier.uri | https://depositonce.tu-berlin.de/handle/11303/7203 | |
| dc.identifier.uri | http://dx.doi.org/10.14279/depositonce-6478 | |
| dc.language.iso | en | |
| dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | |
| dc.subject.ddc | 610 Medizin und Gesundheit | |
| dc.title | Automated universal chip platform for fluorescence based cellular assays | en |
| dc.type | Article | |
| dc.type.version | publishedVersion | |
| dcterms.bibliographicCitation.doi | 10.1515/bmt-2012-4319 | |
| dcterms.bibliographicCitation.issue | Suppl. 1 | |
| dcterms.bibliographicCitation.journaltitle | Biomedical engineering = Biomedizinische Technik | |
| dcterms.bibliographicCitation.originalpublishername | De Gruyter | |
| dcterms.bibliographicCitation.originalpublisherplace | Berlin [u.a.] | |
| dcterms.bibliographicCitation.pageend | 343 | |
| dcterms.bibliographicCitation.pagestart | 340 | |
| dcterms.bibliographicCitation.volume | 57 | |
| tub.accessrights.dnb | domain | |
| tub.affiliation | Fak. 3 Prozesswissenschaften::Inst. Biotechnologie::FG Medizinische Biotechnologie | de |
| tub.affiliation.faculty | Fak. 3 Prozesswissenschaften | de |
| tub.affiliation.group | FG Medizinische Biotechnologie | de |
| tub.affiliation.institute | Inst. Biotechnologie | de |
| tub.publisher.universityorinstitution | Technische Universität Berlin |
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