dc.contributor.author	Martin, Robbie M.
dc.contributor.author	Moniruzzaman, Mohammad
dc.contributor.author	Stark, Gwendolyn F.
dc.contributor.author	Gann, Eric R.
dc.contributor.author	Derminio, Dominique S.
dc.contributor.author	Wei, Bofan
dc.contributor.author	Hellweger, Ferdi L.
dc.contributor.author	Pinto, Ameet
dc.contributor.author	Boyer, Gregory L.
dc.contributor.author	Wilhelm, Steven W.
dc.date.accessioned	2020-12-17T08:25:50Z
dc.date.available	2020-12-17T08:25:50Z
dc.date.issued	2020-12-03
dc.date.updated	2020-12-17T07:08:18Z
dc.description.abstract	Microcystins produced during harmful cyanobacterial blooms are a public health concern. Although patterns are emerging, the environmental cues that stimulate production of microcystin remain confusing, hindering our ability to predict fluctuations in bloom toxicity. In earlier work, growth at cool temperatures relative to optimum (18°C vs. 26°C) was confirmed to increase microcystin quota in batch cultures of Microcystis aeruginosa NIES-843. Here, we tested this response in M. aeruginosa PCC 7806 using continuous cultures to examine temporal dynamics and using RNA-sequencing to investigate the physiological nature of the response. A temperature reduction from 26 to 19°C increased microcystin quota ∼2-fold, from an average of ∼464 ag μm–3 cell volume to ∼891 ag μm–3 over a 7–9 d period. Reverting the temperature to 26°C returned the cellular microcystin quota to ∼489 ag μm–3. Long periods (31–42 d) at 19°C did not increase or decrease microcystin quota beyond that observed at 7–9 d. Nitrogen concentration had little effect on the overall response. RNA sequencing indicated that the decrease in temperature to 19°C induced a classic cold-stress response in M. aeruginosa PCC 7806, but this operated on a different timescale than the increased microcystin production. Microcystin quota showed a strong 48- to 72-h time-lag correlation to mcy gene expression, but no correlation to concurrent mcy expression. This work confirms an effect of temperature on microcystin quota and extends our understanding of the physiological nature of the response.	en
dc.identifier.eissn	1664-302X
dc.identifier.uri	https://depositonce.tu-berlin.de/handle/11303/12227
dc.identifier.uri	http://dx.doi.org/10.14279/depositonce-11102
dc.language.iso	en	en
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	en
dc.subject.ddc	570 Biowissenschaften; Biologie	de
dc.subject.other	microcystin	en
dc.subject.other	temperature	en
dc.subject.other	cyanotoxins	en
dc.subject.other	cyanobacteria	en
dc.subject.other	episodic events	en
dc.title	Episodic Decrease in Temperature Increases mcy Gene Transcription and Cellular Microcystin in Continuous Cultures of Microcystis aeruginosa PCC 7806	en
dc.type	Article	en
dc.type.version	publishedVersion	en
dcterms.bibliographicCitation.articlenumber	601864	en
dcterms.bibliographicCitation.doi	10.3389/fmicb.2020.601864	en
dcterms.bibliographicCitation.journaltitle	Frontiers in Microbiology	en
dcterms.bibliographicCitation.originalpublishername	Frontiers	en
dcterms.bibliographicCitation.originalpublisherplace	Lausanne	en
dcterms.bibliographicCitation.volume	11	en
tub.accessrights.dnb	free	en
tub.affiliation	Fak. 3 Prozesswissenschaften::Inst. Technischen Umweltschutz::FG Wasserreinhaltung	de
tub.affiliation.faculty	Fak. 3 Prozesswissenschaften	de
tub.affiliation.group	FG Wasserreinhaltung	de
tub.affiliation.institute	Inst. Technischen Umweltschutz	de
tub.publisher.universityorinstitution	Technische Universität Berlin	en

Episodic Decrease in Temperature Increases mcy Gene Transcription and Cellular Microcystin in Continuous Cultures of Microcystis aeruginosa PCC 7806

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